Objective:To observe the effect of Fuzheng Qufeng prescription on 24-hour urine protein(24 h-Upro)

serum albumin

serum creatinine

blood urea nitrogen and renal pathological changes in rats with membranous nephropathy

and its clinical effects. Method:The rats were randomly divided into normal group and modeling group. The rat model was induced with bovine serum albumin (C-BSA). Model rats were randomly divided into model group

and the Fuzheng Qufeng prescription group. The Fuzheng Qufeng group was administered by gavage with Fuzheng Qufeng prescription for 4 weeks. Meanwhile

the normal group and model group were administered by gavage with the same volume of deionized water. At the time points of 0

2

4 weeks

24 h-Upro was detected. At the end of the 4th week

the levels of serum albumin (ALB)

blood urea nitrogen (BUN) and serum creatinine (CRE) were detected. Subsequently

rats in each group were put to death

and the renal pathological changes were observed under light microscope with hematoxylin and eosin (HE) staining

Masson staining and periodic acid silver methe-ramine (PASM) staining. Depositions of IgG and C3 were observed by indirect immunofluorescence technique. The thickening of basement membrane and the changes in the podocytic process were observed under electron microscope. Result:Compared with normal group

the level of 24 h-Upro in model group was significantly increased (P<0.01)

whereas the level of ALB was decreased (P<0.01)

with significant pathological changes in renal tissues. Compared with model group

the level of 24 h-Upro in the Fuzheng Qufeng prescription was decreased (P<0.05)

while the level of ALB was increased(P<0.05). The deposition of immune complex

the thickening of basement membrane and the podocytic process were all alleviated dramatically. Conclusion:Fuzheng Qufeng prescription can effectively alleviate albuminuria of rats with membranous nephropathy

increase blood albumin

relieve the renal pathological damage

and delay the progress of membranous nephropathy.