Objective:To explore the drug factors for hepatotoxicity of Zhixue capsule

the uniform design method was used by comparing five different Chinese medicine prescriptions in rats. Method:Wistar rats were randomly divided into blank control group and five Zhixue capsule groups. The rats were administrated with different drugs by gavage for 30 days. After the last drug administration

blood was collected from abdominal aorta

and serum was taken by centrifugation. Aspartate aminotransferase (AST)

alanine aminotransferase (ALT)

alkaline phosphatase (ALP)

total bilirubin (TBIL) and γ-glutamyltransferase (GGT) in serum were detected to indicate the liver injury. The livers were taken for weighing and calculating absolute liver weight and organ coefficient. The largest liver lobe was stained with hematorylin-eosin (HE)

and then pathological examination was performed under the microscope. Result:Compared with normal control group

general conditions of rats of Zhixue capsule groups were good

GGT of Zhixue capsule No.5 was significantly increased (P<0.01)

absolute liver weight and organ coefficient of Zhixue capsule groups No.3

No.4 and No.5 were significantly increased (P<0.05

P<0.01)

and Zhixue capsule ethanol extracts showed a clear hepatotoxicity

and histopathological observation showed small bubble type fatty degeneration surrounding hepatic lobule. According to the regression analysis of the indicators

cortex dictam may be the main drug factors for hepatotoxicity of Zhixue capsule. Conclusion:It is possible to improve the basic data for the safety of traditional Chinese medicine

guide the clinical safety medication

and improve the research and development of traditional Chinese medicine.