Objective:To study on the absorptive characteristics of Inula cappa extract in rats by in situ intestinal circulating perfusion model. Method:Nine representative ingredients in I. cappa extract were selected as objects of this study.An UPLC-MS/MS was established to determine their cumulative absorption amount and investigate the absorptive amount of the nine components at different intestinal segments

influence of extract concentration

pH value

P-glycoprotein inhibitor

bile and different intestinal segments on intestinal absorption of the nine components for expounding the absorptive characteristics of ingredients in different intestinal sections. Result:Scopolin in high concentration existed saturation phenomenon

it indicated the transport mechanism of scopolin was active transport in the intestine and the other 8 compounds were passive diffusion.The best absorption sites of luteolin-7-glucoside

1

3-O-dicaffeoylquinic acid

4

5-O-dicaffeoylquinic acid and 3

4-O-dicaffeoylquinic acid were jejunum.The best absorption sites of scopolin and 3

5-O-dicaffeoylquinic acid were ileum.The best absorption sites of chlorogenic acid

cryptochlorogenic acid and neochlorogenic acid were duodenum.The absorption of all the compounds were affected by pH and bile. Conclusion:All of the nine ingredients in I. cappa extract can be absorbed in small intestine

but their absorption rate

the best absorptive site and mechanism are not the same.