Objective:The chemical constituents of n-butanol and petroleum ether were separated and the compounds were identified from Millettla nitida var. hirsutissima. Method:The n-butanol and petroleum ether fractions from M. nitida var. hirsutissima were isolated by macroporous adsorption resin chromatography

silica gel column

re-crystallization

Sephadex LH-20 column

reversed phase high-performance liquid chromatography and identified on physicochemical properties and spectral evidences. Result:Finally 15 compounds were isolated from n-butanol and petroleum ether fractions from M. nitida var. hirsutissima

including one neolignan

(+)-lyoniresinol-3α-O-D-glucopyranoside (1); two triterpenoids

taraxeron (2)

epilupeol (3); and twelve flavonoids

biochanin A 7-O-β-D-glucopyranoside (4)

formononetin-7-O-β-D-apiofuranosyl-(1→6)-O-β-D-glucopyranoside (5)

sphaerobioside (6)

8-methylretusin-7-O-β-D-glucopyranoside (7)

afromosin 7-O-β-D-glucopyranoside (8)

β-galactosyl 1-6β-galactosyl formononetin (9)

retusin 8-methyl ether (10)

7-hydroxy-4'

6-dimethoxyisoflavone (11)

4'-methoxy-7-hydroxyisoflavone(12)

biochanin A-7-O-β-D-glucoside(13)

lanceolarin(14)

and 6-methoxyisoformononetin (15). Conclusion:Compounds 1

3

4

7

9

10 and 15 were isolated from this the genus Millettia for the first time

and compounds 1

2

3

4

7

9

10 and 15 were obtained from this plant for the first time

providing chemical basis and foundation for the comprehensive development and the search for active substances of natural plant.