Objective:The research group found in the previous studies that the dichloromethane and ethyl acetate extracts of Siegesbeckia glabrescens had significant physiological activity to inhibit necroptosis when screening the self-built sample library of 100 kinds of traditional Chinese medicines in Jiangxi. In order to identify the active components and find the target compounds

the dichloromethane and ethyl acetate extracts of S. glabrescens were isolated and studied systemically in chemistry. Method:The compounds were isolated and purified by chromatography on silica gel (dichloromethane-methanol

20:1-0:1)

ODS (30%-100% methanol)

Sephadex LH-20 column and recrystallization

and their structures were determined according to physicochemical properties and spectral analyses. Result:Nine compounds were isolated and identified as 2-methoxy-4-(2-propen-1-yl)penyl-6-acetate-β-D-glucopyranoside (1)

benzyl-β-D-glucopyranoside (2)

coumarin (3)

palmitic acid (4)

ethyl caffeate (5)

trans-p-hydroxycinnamic acid (6)

β-sitosterol (7)

β-daucosterol (8) and stigmasterol (9). Conclusion:Compounds 1-6 were isolated from this plant for the first time.