金荞麦提取物对肠易激综合征大鼠肥大细胞,PAR-2,SP的影响

严晶; 刘丽娜; 陆茵; 尚文斌; 鲁俊; 葛飞; 余利华; 彭雯; 于希忠; 张颢; 赵娟; 孙志广

doi:10.13422/j.cnki.syfjx.2018020092

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金荞麦提取物对肠易激综合征大鼠肥大细胞,PAR-2,SP的影响

药理 | 更新时间：2024-01-04

- 金荞麦提取物对肠易激综合征大鼠肥大细胞,PAR-2,SP的影响
- Effect of Fagopyri Dibotryis Rhizoma Extracts on Mast Cells Protease Activated Receptor 2 and Substance P in Rat Models of Irritable Bowel Syndrome
- 中国实验方剂学杂志 2018年24卷第2期页码：92-97
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金青年基金项目（81503536，81603578）;江苏省自然科学基金青年基金项目（BK20151008，BK20140959）;高等学校博士学科点专项科研基金联合项目（20133237110008）;江苏省高校自然科学研究面上项目（15KJB360008）
- DOI：10.13422/j.cnki.syfjx.2018020092
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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严晶, 刘丽娜, 陆茵, 等. 金荞麦提取物对肠易激综合征大鼠肥大细胞,PAR-2,SP的影响[J]. 中国实验方剂学杂志, 2018,24(2):92-97.

YAN Jing, LIU Li-na, LU Yin, et al. Effect of Fagopyri Dibotryis Rhizoma Extracts on Mast Cells Protease Activated Receptor 2 and Substance P in Rat Models of Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 92-97.
严晶, 刘丽娜, 陆茵, 等. 金荞麦提取物对肠易激综合征大鼠肥大细胞,PAR-2,SP的影响[J]. 中国实验方剂学杂志, 2018,24(2):92-97. DOI： 10.13422/j.cnki.syfjx.2018020092.

YAN Jing, LIU Li-na, LU Yin, et al. Effect of Fagopyri Dibotryis Rhizoma Extracts on Mast Cells Protease Activated Receptor 2 and Substance P in Rat Models of Irritable Bowel Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(2): 92-97. DOI： 10.13422/j.cnki.syfjx.2018020092.

摘要

目的：观察金荞麦提取物（Fag）对肠易激综合征（IBS）模型大鼠内脏高敏感的作用以及对肥大细胞（MC），蛋白酶激活受体-2（PAR-2），P物质（SP）的影响。方法：将清洁级新生雄性乳大鼠采用新生期母婴分离、乙酸灌肠、鸡卵清白蛋白腹腔注射的复合造模方法制备IBS内脏高敏感模型，大鼠随机分为正常组（生理盐水），模型组（生理盐水），Fag低、中、高剂量组（0.14，0.42，1.26 g·kg-1），酮替芬组（0.18 mg·kg-1），每组10只，连续灌胃14 d。采用腹壁撤退反应（AWR）评估大鼠内脏敏感性，免疫组化法（immunohistochemistry）检测大鼠结肠MC数量、脱颗粒程度，蛋白免疫印迹法（Western blot）检测PAR-2蛋白表达，免疫组化、酶联免疫吸附法（ELISA）检测大鼠结肠SP水平。结果：与正常组比较，模型组20，40，60 mmHg（1 mmHg=0.133 kPa）压力下AWR评分明显升高，结肠MC数量，脱颗粒程度，PAR-2，SP表达明显上升（P<0.05）；与模型组比较，Fag中剂量组（20，40，60 mmHg压力下），Fag高剂量组（20，40 mmHg压力下）的AWR评分，远端结肠MC数量，脱颗粒程度，PAR-2，SP表达明显下降（P<0.05）；与酮替芬组比较，Fag高剂量组AWR评分（40 mmHg压力下），SP水平降低（P<0.05）。结论：Fag能降低IBS模型大鼠内脏敏感性，其机制与抑制MC数量及活化脱颗粒程度，下调PAR-2受体，降低SP有关，并且镇痛作用优于酮替芬。

Abstract

Objective:To observe the effect of Fagopyri Dibotryis Rhizoma extracts (Fag) on mast cell (MC)

protease activated receptor-2 (PAR-2) and substance P (SP) in irritable bowel syndrome (IBS) rat models with visceral hypersensitivity. Method:IBS rat models were established through neonatal maternal separation

acetic acid enema and intraperitoneal injection with OVA. A total of 60 SD rats were randomly divided into normal group (normal saline)

model group (normal saline)

low

middle and high-dose Fag groups (0.14

0.42

1.26 g·kg-1) and ketotifen group (0.18 mg·kg-1). After oral gavage for 14 days

visceral hypersensitivity was assessed based on abdominal withdrawal reflex (AWR) score. The number and degranulation of mast cells were observed by immumohistochemical staining(IHC); PAR-2 was measured by Western blot; and SP was evaluated by enzyme linked immunoabsorbent assay and IHC. Result:AWR score in model group was significantly higher than that of control group at the distension pressures of 20

60 mmHg(1 mmHg=0.133 kPa); and the number and degranulation of mast cells

PAR-2 and SP expressions significantly increased. Middle-dose Fag group (at the distension pressures of 20

60 mmHg) and high-dose Fag group (at the distension pressures of 20

40 mmHg) could effectively reduce AWR scores. And the number and degranulation of mast cells

PAR-2 and SP expressions were lower than those of model group. Furthermore

at the distension pressure of 40 mmHg

high-dose Fag group could lower than ketotifen-treated group in terms of the AWR score. Conclusion:Fag could alleviate the visceral hypersensitivity in IBS rat models. Its mechanism was related with the inhibition of the number and degranulation of intestinal mucosal mast cells

the down-regulation of PAR-2 and the reduction of SP.

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