不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分的差异及相关性分析

戴雪雯; 冯丽丽; 李海峰

doi:10.13422/j.cnki.syfjx.2018030041

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不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分的差异及相关性分析

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- 不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分的差异及相关性分析
- Analysis of Differences and Correlation of Steroidal Saponins in Rhizomes and Leaves of var. from Different Planting Base
- 中国实验方剂学杂志 2018年24卷第3期页码：41-48
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81360616）
- DOI：10.13422/j.cnki.syfjx.2018030041
  中图分类号： R284.1
- 纸质出版日期：2018
- 稿件说明：
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戴雪雯, 冯丽丽, 李海峰. 不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分的差异及相关性分析[J]. 中国实验方剂学杂志, 2018,24(3):41-48.

DAI Xue-wen, FENG Li-li, LI Hai-feng. Analysis of Differences and Correlation of Steroidal Saponins in Rhizomes and Leaves of var. from Different Planting Base[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 41-48.
戴雪雯, 冯丽丽, 李海峰. 不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分的差异及相关性分析[J]. 中国实验方剂学杂志, 2018,24(3):41-48. DOI： 10.13422/j.cnki.syfjx.2018030041.

DAI Xue-wen, FENG Li-li, LI Hai-feng. Analysis of Differences and Correlation of Steroidal Saponins in Rhizomes and Leaves of var. from Different Planting Base[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(3): 41-48. DOI： 10.13422/j.cnki.syfjx.2018030041.

摘要

目的：探讨不同种植基地滇重楼根茎和叶中主成分及甾体皂苷类有效成分的差异及相关性。方法：采用液质联用技术（LC-MS）定性分析不同种植基地滇重楼根茎和叶中甾体皂苷类有效成分、主成分的差异及相关性，流动相水（A）-乙腈（B）梯度洗脱（0~12 min，45%~60% B；12~25 min，60%~30% B；25~28 min，30%~100% B；28~30 min，100% B；30~35 min，100%~45% B；35~40 min，45% B），检测波长203 nm，采用电喷雾离子源（ESI），正离子模式扫描，毛细管电压4 kV，终板抵消电压500 V，雾化器压力253.8 kPa，干燥气体为氮气，气体流速8 L·min-1，干燥温度350℃，离子源温度220℃。结果：定性检测到不同种植基地滇重楼根茎和叶中重楼皂苷Ⅰ，重楼皂苷Ⅱ，重楼皂苷Ⅲ（薯蓣皂苷），重楼皂苷Ⅴ，重楼皂苷Ⅵ，重楼皂苷Ⅶ，重楼皂苷H，纤细薯蓣皂苷8种甾体皂苷有效成分；重楼皂苷Ⅰ，重楼皂苷Ⅲ（薯蓣皂苷），重楼皂苷Ⅴ，重楼皂苷Ⅶ是不同种植基地滇重楼根茎中的主要存在形式，重楼皂苷Ⅴ和重楼皂苷Ⅵ是不同种植基地滇重楼叶中的主要存在形式；根据不同种植基地滇重楼根茎、叶正离子模式下液质数据的主成分分析模型可将其分为4类，其中巍山、南涧、弥渡、曲靖4个种植基地滇重楼根茎中主成分和不同种植基地滇重楼叶中主成分聚为一类。结论：不同种植基地滇重楼根茎和叶中8种主要甾体皂苷类有效成分存在较大差异；不同种植基地滇重楼根茎中主成分差异较大，而叶中主成分无显著差异。

Abstract

Objective:To investigate the differences and correlations of steroidal saponins and principal components in rhizomes and leaves of Paris polyphylla var. yunnanensis from different planting base. Method:The differences and correlations of steroidal saponins and principal components in the rhizomes and leaves of P. polyphylla var. yunnanensis from different planting base were qualitative analysis by LC-MS. Result:PolyphyllinⅠ

polyphyllin Ⅱ

polyphyllin Ⅲ(dioscin)

polyphyllin Ⅴ

polyphyllin Ⅵ

polyphyllin Ⅶ

polyphyllin H and gracillin were detected in the rhizomes and leaves of P. polyphylla var. yunnanensis from different planting base;polyphyllin Ⅰ

polyphyllin Ⅲ(dioscin)

polyphyllin Ⅴ and polyphyllin Ⅶ were the main existing forms of steroidal saponins in the rhizomes of P. polyphylla var. yunnanensis from different planting base;polyphyllin Ⅴ and polyphyllin Ⅵ were the main existing forms of steroidal saponins in the leaves of P. polyphylla var. yunnanensis from different planting base;it can be classified into four categories according to the principal component analysis(PCA) model of the LC-MS data under the positive ion mode of rhizomes and leaves of P. polyphylla var. yunnanensis from different planting base

the principal component in rhizomes of P. polyphylla var. yunnanensis from Weishan

Nanjian

Midu and Qujing was classified into the same category with principal component in the leaves of samples from different planting base. Conclusion:There are significant differences of eight main steroidal saponins in the rhizomes and leaves of P. polyphylla var. yunnanensis from different planting base;there is significant differences of principal component in the rhizomes of P. polyphylla var. yunnanensis from different planting base

but there is little differences in the leaves.

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