Objective:To provide scientific evidence for development and utilization of Eureka lemon flower so as to effectively make use of lemon and promote the industry chain by analyzing and identifying the chemical constituents of essential oil by GC-MS. Method:The essential oil of Eureka lemon flower was extracted by steam distillation and the oil yield was calculated. The oil sample was analyzed by GC-MS

and the chemical components were identified by combining mass NIST 14.0 spectrometry database with retention index (RI) and documents

then the peak area normalization method was used to calculate the relative percentage of each ingredient. Result:The essential oil yield was 0.36%. A total of 76 components were detected and 64 components were identified

accounting for 98.94% of the total constituents. The chemical compositions from lemon flower volatile oil mainly included olefin (28) 81.90%

alcohol (14) 12.28%

aldehydes (6) 3.34%

esters (5) 0.45%

and alkane (6) 0.79%

etc. Its major components included d-limonene(59.88%)

β-pinene(5.52%)

(Z)-β-ocimene(5.33%)

trans-nerolidol(4.84%)

3

7

11-trimethyl-6

10-dodecadien-1-ol(4.49%)

γ-terpinene(4.11%)

etc. Conclusion:Most components of flower essential oil were similar to those in the leaves

which both include d-limonene

β-pinene and other active constituents. Beside that

the content of d-limonene (59.88%) was much higher than that in the literature of lemon leaves (17.22%)

indicating that Eureka lemon flowers have potential exploitation value.