Objective:To establish the flavonoids fingerprints of one or two years old Hedysari Radix and Astragali Radix from different areas in Gansu province

provide reference for scientific evaluation of the intrinsic quality of Gansu Hedysari Radix and Astragali Radix

and clarify the basis on whether Hedysari Radix and Astragali Radix can be alternatively used. Method:HPLC was used to determine the flavonoids fingerprints of 28 batches of one or two years Hedysari Radix and 28 batches of one or two years Astragali Radix in Gansu. Chromatographic conditions:Agilent ZROBAX-C18

mobile phase acetonitrile-0.1% formic acid aqueous solution

gradient elution

flowrate 1.0 mL·min-1

detection wavelength 254 nm

and column temperature 35℃. Result:The flavonoids fingerprint showed 19 common peaks in one or two years old Hedysari Radix

and 14 common peaks in one or two years Astragali Radix

9 common peaks in one year old Astragali Radix and Hedysari Radix

and 8 common peaks in two years old Astragali Radix and Hedysari Radix. Among them

the average similarity was 0.976 in one year Hedysari Radix from different areas

0.888 in two years Hedysari Radix

0.986 in one year Astragali Radix from different areas

and 0.911 in two years Astragali Radix; the similarity was 0.534 between one year Hedysari Radix and Astragali Radix

and 0.490 between two years Hedysari Radix and Astragali Radix. Conclusion:The method is reproducible and the established fingerprints provide the basis for the quality control and evaluation of Gansu Hedysari Radix and Astragali Radix. The similarity of Hedysari Radix and Astragali Radix in Gansu was very low

and the similarity decreased with the increase of age

so these two samples are not suitable for alternative use.