Objective:To study the effect of Jianpi Gushen Huayu decoction (JGHD) in alleviating and protecitng renal tissue rats with diabetic nephropathy. Method:Totally 60 male SD rats were randomly divided into 6 groups:blank group

model group

ramipril group

low-dose JGHD group

middle-dose JGHD group

and high-dose JGHD group. After modeling

triglyceride

total cholesterol

creatinine

urea and 24-hour urine microalbumin were observed in 6 groups of rats. The morphological changes in kidney were observed under light microscope. Immunohistochemical staining was used to observe transforming growth factor-β1 (TGF-β1)

metallothionein-1 (MT-1)

α-smooth muscle actin (α-SMA)

light chain-Ⅱ(LC3-Ⅱ)

and autophagy-related protein Beclin. TGF-β1 mRNA expression was detected by reverse transcription PCR (RT-PCR) in rats. Result:Jianpi Gushen Huayu decoction could reduce the levels of serum triglyceride

total cholesterol

creatinine

urea

24 h urinary albumin

and effectively inhibit levels of TGF-β1

MT-1

α-SMA

LC3-Ⅱ

Beclin and TGF-β1 mRNA expression. It could improve the pathological changes in renal tissues. Conclusion:Jianpi Gushen Huayu decoction could reduce serum triglyceride

total cholesterol

creatinine

urea

24 h urinary microalbumin

TGF-β1

MT-1

α-SMA

LC3-Ⅱ

Beclin and TGF-β1 mRNA expression

with the effect in alleviating and protecting the pathological changes in renal tissues.