基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异

王静; 陈悦; 袁子民; 贾天柱

doi:10.13422/j.cnki.syfjx.2018040008

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基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异

药剂与炮制 | 更新时间：2024-01-04

- 基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异
- Differences Studied on Mechanism of Long-term Toxicity in Rats of Myristicae Semen After and Before Being Processed with Wheat Bran Based on Urinary Metabonomics
- 中国实验方剂学杂志 2018年24卷第4期页码：8-13
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81274084）;辽宁省自然科学基金项目（201602511）
- DOI：10.13422/j.cnki.syfjx.2018040008
  中图分类号： R283;R285.5
- 纸质出版日期：2018
- 稿件说明：
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王静, 陈悦, 袁子民, 等. 基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异[J]. 中国实验方剂学杂志, 2018,24(4):8-13.

Wang Jing, CHEN Yue, YUAN Zi-min, et al. Differences Studied on Mechanism of Long-term Toxicity in Rats of Myristicae Semen After and Before Being Processed with Wheat Bran Based on Urinary Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 8-13.
王静, 陈悦, 袁子民, 等. 基于尿液代谢组学分析肉豆蔻麸煨炮制前后对大鼠长期毒性的作用差异[J]. 中国实验方剂学杂志, 2018,24(4):8-13. DOI： 10.13422/j.cnki.syfjx.2018040008.

Wang Jing, CHEN Yue, YUAN Zi-min, et al. Differences Studied on Mechanism of Long-term Toxicity in Rats of Myristicae Semen After and Before Being Processed with Wheat Bran Based on Urinary Metabonomics[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 8-13. DOI： 10.13422/j.cnki.syfjx.2018040008.

摘要

目的：研究肉豆蔻麸煨炮制前后对大鼠长期毒性作用机制的差异性，探讨肉豆蔻麸煨炮制的科学内涵。方法：采用尿液代谢组学法，大鼠随机分为正常组、肉豆蔻生品组（剂量10 g·kg-1）和麸煨品组（剂量10 g·kg-1），灌胃给药12周后，采集各组大鼠24 h的尿样，通过偏最小二乘法-判别分析（PLS-DA）鉴别各组代谢物谱的差异，通过变量重要性投影（VIP）发现并鉴定潜在的生物标志物。结果：由PLS-DA得分可知，生品组与正常组完全分离，麸煨品组位于二者之间，接近于正常组。鉴定了17个与肉豆蔻毒性相关的生物标志物，与正常组比较，生品组中与肾毒性相关的肌酐含量显著降低，与肝毒性相关的尿中泛酸、肉毒碱C2：0和氨基酸代谢异常，麸煨品组对肌酐、泛酸、肉毒碱C2：0和氨基酸代谢均有向正常组显著回调的作用。结论：肉豆蔻生品长期应用有致肝、肾毒性，而麸煨炮制可降低该毒性。

Abstract

Objective: To develop the differences on mechanism of long-term toxicity in rats treated with raw products and processed products with wheat bran of Myristicae Semen

and to explore the connotation about its processing technology with wheat bran. Method: A urinary metabonomics method was developed to study on long-term toxicity in rats of Myristicae Semen before and after being processing

rats were divided into three groups

including normal group

the raw products group and the processed products group with the dose of 10 g·kg-1 for 12 weeks.Urinary samples of 24 hours after stopping the intragastric administration were collected.Partial least squares-discriminant analysis (PLS-DA) was employed to process data from all the groups

variable importance in the projection (VIP) were adopted to analyze potential biomarkers. Result: Good separation was observed between the raw products group and the normal group

the prcessed products group lied between the two groups

and was close to the normal group.Seventeen potential biomarkers related with long-term toxicity were screened out.Compared with those in the normal group

creatinine in the urine associated with renal toxicity significantly decreased in the raw products group;pantothenic acid

carnitine C2: 0 and amino acids in the urine associated with hepatotoxicity metabolized abnormally;these biomarkers in the processed products group were adjusted significantly to the normal group. Conclusion: When large amounts of the raw Myristicae Semen are taken for a long time

hepatic and renal toxicity will occur

but its toxic effects can decrease significantly after being processed with wheat bran.

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