Objective: To study the chemical constituents of Artocarpus nigrifolius of Artocarpus genus (Moraceae). Method: Compounds were isolated and purified by the normal phase silica gel

MCI gel

Sephadex LH-20

ODS

HPLC and other column chromatography separation methods. Their structures were identified by spectrascopic methods based on physicochemical properties. Result: Twenty-six compounds

including 16 triterpenoids

2 nortriterpenoids

6 steroids

1 benzaldehyde derivative and 1 amino compound

were isolated from the ethyl acetate fraction in 95% ethanol extract of Artocarpus nigrifolius. All of the above isolates were identified as 20-hydroxy-4α

4β

14α-trimethyl-9β

19-cyclopregnane-3-one (1)

1-(tert-butylamino)propan-2-one (2)

24-methylenecycloartanone (3)

cyclolaudenone (4)

24-methylenecycloartanol (5)

4-hydroxy-3

5-dimethxybenzaldehyde (6)

cycloeucalenol (7)

cycloart-23-ene-3β

25-diol (8)

β-sitosterol (9)

3β

24

25-trihydroxycycloartane (10)

(23E)-27-nor-3α-hydroxycycloart-23-en-25-one (11)

3β-hydroxy-22

23

24

25

26

27-hexanordammarane-20-one (12)

12-en-3β-hydroxy-olean-11-one (13)

(24S)-cycloartane-24

25-diol-3-one (14)

betulinic acid (15)

lupeol (16)

α-amyrin (17)

(22E)-25

26

27-trinor-3β-hydroxycycloart-22-en-24-al (18)

3β-hydroxy-urs-11-en-13β

28-olide (19)

11α-hydroxy-α-amyrin (20)

(22E)-3β-hydroxystigmasta-5

22-dien-7-one (21)

7-ketositosterol (22)

β-amyrin (23)

(24S)-methyl-5-cholestene-3β

7α-diol (24)

7α-hydroxysitosterol (25) and 7β-hydroxysitosterol (26)

respectively. Conclusion: Compound 1 is a novel cycloartane C24 nortriterpenoid

and compound 2 is reported as a naturally occurring compound for the first time. Compounds 6

8

11

15-17

20

22 and 23 are isolated from Artocarpus genus for the first time. Besides

all of the other compounds were isolated from Artocarpus genus for the first time. Triterpenoids may be the antitumor active components of A. nigrifolius

and is worth further study.