Objective: To observe the expressions of basic fibroblast growth factor (bFGF)

serine/threonine kinase (Akt) and cystein-asparate protease-9 (Caspase-9) in diabetic rat ulcer granulation tissues

in order to investigate the mechanism of Shengji Xiangpi mastic in promoting the ulcer healing in diabetic rats. Method: The ulcer model of type 2 diabetic SD rats was established

and randomly divided into normal saline group

vaseline group and Shengji Xiangpi mastic group. The ulcer model of normal rats was set up as the blank control group. The rats in Shengji Xiangpi mastic group were applied with Shengji Xiangpi mastic and covered with gauze

those in vaseline group were applied with vaseline and covered with gauze

and those in normal saline group and blank control group were treated with physiological saline. The wound healing conditions in each group were observed on 1

3

7

14

21 d after modeling

and ulcer tissues were collected. Hematoxylin-eosin (HE) staining was performed to observe the morphological changes in wound granulation tissues. Real-time PCR and Western blot were used to analyze the expression of bFGF

emzyme-linked immunosorbent assay(ELISA) was used to analyze the expression of p-Akt

and immunohistochemistry was used to analyze the expression of Caspase-9. Result: Compared with vaseline group and saline group

Shengji Xiangpi mastic significantly up-regulated bFGF expression in ulcer granulation tissues of diabetic rats

increased p-Akt content

and inhibited the expression of Caspase-9. Conclusion: Shengji Xiangpi mastic can promote ulcer healing in diabetic rats through bFGF/Akt/Caspase pathway.