Objective: To study the effect of Dracaenae Cochinchinensis Resina on experimental tree shrews' myocardial ischemia reperfusion model in vitro. Method: The experimental tree shrews' myocardial ischemia reperfusion model was established by the Langendorff isolated heart perfusion system. This study contained 6 groups

namely blank group

model group

solvent control group and low-dose

medium-dose

high-dose Dracaenae Cochinchinensis Resina (0.25

0.5

1.0 g·L-1) groups. The hearts were collected and stored at -20 ℃ after reperfusion. Alanine aminotransferase (ALT)

aspartate transaminase (AST) and lactic dehydrogenase (LDH) were measured by enzyme-labeled immunosorbent assay. Creatine Kinase MB (CK-MB) was measured by immunosuppression; Superoxide dismutase (SOD) was measured by xanthinoxidase method; malondialdehyde was measured by thiobarbital staining method; infarct size was measured by 2

3

5-triphenyltrazoliumchloride (TTC) method

and apoptotic rate was measured by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) method. Result: After reperfusion with Dracaenae Cochinchinensis Resina

CK-MB

LDH

ALT

AST in perfusion fluid and tissues significantly reduced

activity of SOD increased

the myocardial infarction area and TUNEL apoptosis rate reduced. Conclusion: Reperfusion with Dracaenae Cochinchinensis Resina had effect against to myocardial ischemia-reperfusion injury by inhibiting the production of excess oxygen free radicals

meanwhile decreasing myocardial infarction area and apoptosis rate.