DING Ge, ZHANG Dai-zhen, DING Xiao-yu. Molecular Level of Resources[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 208-215.
DING Ge, ZHANG Dai-zhen, DING Xiao-yu. Molecular Level of Resources[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(4): 208-215. DOI: 10.13422/j.cnki.syfjx.2018040208.
Dendrobium has significant effects in nourishing yin and generating body fluid
benefiting lung and eyes
resisting cancer and prolonging life
thus with a wide distribution and a long history of application. With the development of molecular biological technology
studies on Dendrobium medicinal resources have advanced rapidly. DNA marker technology has the characteristics of micro-scale and high velocity
specificity
accuracy and credibility
and provides a new method for studies on Dendrobium resources. The genetic diversity and genetic relationship among different species of Dendrobium were analyzed based on combined use of RAPD
ISSR
AFLP
SRAP and other molecular markers. The genetic information obtained from these studies could be used for the conservation and development of Dendrobium resources. The combined application of nuclear gene
mitochondrial gene and chloroplast gene can identify Dendrobium effectively and accurately
and provide scientific basis for the further development of specific molecular identification of traditional Chinese medicinal herbs. At the same time
the combined use of different gene sequences can be taken as molecular markers of DNA barcode of Dendrobium
which will provide new ideas and methods for the identification of Dendrobium herbs
and improve the identification speed and efficiency of Dendrobium species effectively. In this paper
molecular identification
DNA sequences
functional genes of Dendrobium genus were discussed
in order to provide the theoretical basis for the further development and application of the medicinal plants
as well as scientific basis for the effective protection and sustainable development of Dendrobium.