温度、光照及pH对蟾酥提取液中指标成分稳定性的影响

李国雁; 陈灵; 肖丹; 彭衡阳; 王森

doi:10.13422/j.cnki.syfjx.2018050031

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温度、光照及pH对蟾酥提取液中指标成分稳定性的影响

药剂与炮制 | 更新时间：2024-01-04

- 温度、光照及pH对蟾酥提取液中指标成分稳定性的影响
- Effect of Temperature,Light and pH on Stability of Index Components in Extract of Bufonis Venenum
- 中国实验方剂学杂志 2018年24卷第5期页码：31-35
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省药剂学研究生卓越人才培养计划项目（黔教研合ZYRC字[2014]019）
- DOI：10.13422/j.cnki.syfjx.2018050031
  中图分类号： R284.1
- 纸质出版日期：2018
- 稿件说明：
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李国雁, 陈灵, 肖丹, 等. 温度、光照及pH对蟾酥提取液中指标成分稳定性的影响[J]. 中国实验方剂学杂志, 2018,24(5):31-35.

LI Guo-yan, CHEN Ling, XIAO Dan, et al. Effect of Temperature,Light and pH on Stability of Index Components in Extract of Bufonis Venenum[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 31-35.
李国雁, 陈灵, 肖丹, 等. 温度、光照及pH对蟾酥提取液中指标成分稳定性的影响[J]. 中国实验方剂学杂志, 2018,24(5):31-35. DOI： 10.13422/j.cnki.syfjx.2018050031.

LI Guo-yan, CHEN Ling, XIAO Dan, et al. Effect of Temperature,Light and pH on Stability of Index Components in Extract of Bufonis Venenum[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 31-35. DOI： 10.13422/j.cnki.syfjx.2018050031.

摘要

目的：考察温度、光照及pH对蟾酥提取液中华蟾酥毒基、脂蟾毒配基、总蟾毒色胺稳定性的影响。方法：采用HPLC测定蟾酥提取液中华蟾酥毒基和脂蟾毒配基的含量，检测波长296 nm，流动相乙腈-水（58：42）；利用UV测定蟾酥提取液中总蟾毒色胺的含量，检测波长559 nm。分别考察高温（60，40℃），强光照射[（4 500±500） Lx]及不同pH（40℃，pH分别为4.32，4.99，5.73，6.47，6.97，7.75）条件下蟾酥提取液放置0，5，10 d后华蟾酥毒基、脂蟾毒配基、总蟾毒色胺的含量变化。结果：60℃条件下放置10 d后3种指标成分降解率均>5%；40℃及（4 500±500） Lx条件下华蟾酥毒基和脂蟾毒配基的降解率均<5%；总蟾毒色胺在上述条件下的降解率均>5%。华蟾酥毒基、脂蟾毒配基、总蟾毒色胺分别在pH 5.73，6.47，6.47时降解最慢。结论：蟾酥提取液中华蟾酥毒基和脂蟾毒配基受高温及强光照射的影响较小，总蟾毒色胺受高温及强光照射的影响较大；pH是影响蟾酥提取液中3种指标成分稳定性的主要因素，不同pH条件对3种指标成分的稳定性有不同程度的影响。

Abstract

Objective:To investigate the influence of temperature

light and pH on stability of cinobufagin

resibufogenin and total bufotenine in Bufonis Venenum extract. Method:The contents of cinobufagin and resibufogenin were determined by HPLC

the detection wavelength was 296 nm and the mobile phase was acetonitrile-water(58:42).The content of total bufotenine was determined by UV

the detection wavelength was set at 559 nm.The effect of high temperature (60

40℃)

strong light irradiation[(4 500±500) Lx] and different pH (40℃

pH of 4.32

4.99

5.73

6.47

6.97

7.75) on stability of cinobufagin

resibufogenin and total bufotenine in Bufonis Venenum extract placed 0

10 d was investigated. Result:The degradation of the three indicators was greater than 5% under 60℃ for 10 d.The degradations of cinobufagin and resibufogenin were both <5% under the conditions of 40℃ and (4 500±500) Lx

the degradation of total bufotenine was >5% under the above conditions.Degradation of cinobufagin at pH 5.73 was the slowest

degradation of resibufogenin was the slowest at about pH 6.47

and degradation of total bufotenine at pH 6.47 was the slowest. Conclusion:High temperature and strong light irradiation have small effect on cinobufagin and resibufogenin in Bufonis Venenum extract

but total bufotenine is affected by high temperature and strong light irradiation.The pH is the main factor affecting the stability of three index components in Bufonis Venenum extract

but different pH conditions have different effects on the stability of these three index components.

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