麻杏石甘汤对流感病毒肺部感染模型肺、结肠组织MCP-1蛋白表达水平的影响

邹莉; 何谷良; 卢芳国; 李玲; 张波; 戴冰; 魏科; 陈山泉; 宁毅; 胡珏; 吴涛

doi:10.13422/j.cnki.syfjx.2018050100

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麻杏石甘汤对流感病毒肺部感染模型肺、结肠组织MCP-1蛋白表达水平的影响

药理 | 更新时间：2024-01-04

- 麻杏石甘汤对流感病毒肺部感染模型肺、结肠组织MCP-1蛋白表达水平的影响
- Effect of Maxing Shigan Tang on Protein Expression Level of MCP-1 in Lung Tissue and Colon Tissue Based on Influenza Virus Infection Lung Model
- 中国实验方剂学杂志 2018年24卷第5期页码：100-106
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81774126，81473468，81503445，81703919）;湖南省自然科学基金项目（2016JJ2095，2017JJ3232）;湖南省教育厅创新平台开放基金项目（17K067）;湖南省卫生计生委科研课题项目（C2015-16，C2016049）;湖南省高校科技创新团队项目（15）;湖南省省级精品课程项目（48）;湖南中医药大学重点学科项目（1）
- DOI：10.13422/j.cnki.syfjx.2018050100
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
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邹莉, 何谷良, 卢芳国, 等. 麻杏石甘汤对流感病毒肺部感染模型肺、结肠组织MCP-1蛋白表达水平的影响[J]. 中国实验方剂学杂志, 2018,24(5):100-106.

ZOU Li, HE Gu-liang, LU Fang-guo, et al. Effect of Maxing Shigan Tang on Protein Expression Level of MCP-1 in Lung Tissue and Colon Tissue Based on Influenza Virus Infection Lung Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 100-106.
邹莉, 何谷良, 卢芳国, 等. 麻杏石甘汤对流感病毒肺部感染模型肺、结肠组织MCP-1蛋白表达水平的影响[J]. 中国实验方剂学杂志, 2018,24(5):100-106. DOI： 10.13422/j.cnki.syfjx.2018050100.

ZOU Li, HE Gu-liang, LU Fang-guo, et al. Effect of Maxing Shigan Tang on Protein Expression Level of MCP-1 in Lung Tissue and Colon Tissue Based on Influenza Virus Infection Lung Model[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 100-106. DOI： 10.13422/j.cnki.syfjx.2018050100.

摘要

目的：从流感病毒肺部感染模型肺、结肠组织单核细胞趋化因子（MCP）-1蛋白表达水平，探讨流行性感冒"肺病及肠"的分子机制与麻杏石甘汤的干预作用。方法：经鼻腔接种建立流感病毒小鼠肺部感染模型。实验设正常组、模型组、麻杏石甘汤组和奥司他韦组。经灌胃给药或生理盐水3，7 d后，常规法检测小鼠体质量、肺指数、肺组织和结肠组织病理变化；蛋白免疫印迹法（Western blot）和免疫组化法检测小鼠肺、结肠组织MCP-1蛋白表达水平。结果：①与正常组比较，模型组肺指数显著升高（P<0.01），给药3，7 d的麻杏石甘汤组肺指数显著下降（P<0.01）。②与正常组比较，模型组肺组织、结肠组织炎症充血严重，给药3，7 d的麻杏石甘汤组肺、结肠组织炎症明显减轻。③与正常组比较，模型组肺组织中MCP-1蛋白表达水平显著升高（P<0.01），给药3，7 d的麻杏石甘汤组MCP-1蛋白表达水平显著下降（P<0.01），Western blot和免疫组化法检测结果趋势一致。④与正常组比较，免疫组化法检测结果中的模型组结肠组织中MCP-1蛋白表达水平显著升高（P<0.05），给药3，7 d的麻杏石甘汤组MCP-1表达水平没有明显差异；⑤麻杏石甘汤对流感病毒肺部感染模型肺指数抑制作用与其对肺、结肠组织MCP-1的蛋白表达水平的调节作用呈正相关。结论：流感病毒肺部感染模型伴有严重的肠组织病理损伤以及肺、肠组织中MCP-1的蛋白表达水平的上升，麻杏石甘汤可能通过调节MCP-1蛋白表达水平而实现抗流感病毒作用。

Abstract

Objective:To discuss the molecular mechanism of ‘lung disease transferring to large intestine’ and the intervention effect of Maxing Shigan Tang thought the influenza virus infection lung model and the protein expression level of monocyte chemotactic protein-1 (MCP-1) in lung tissue and colon tissue. Method:Nasal inoculation with influenza virus was performed to establish influenza virus-infected animal models. The experiment included the normal control group

the model control group

the Maxing Shigan Tang group and the oseltamivir group. The groups were given the corresponding drugs or saline by gavage for 3 days and 7 days respectively. The mice's body weight

lung index

pathological changes in lung tissue and colon tissue were detected by routine method; MCP-1 protein expressions in lung tissue and colon tissue were detected by Western blot technology and immunohistochemistry. Result:① Compared with the normal control group

the lung index of the model control group increased

with significant differences (P<0.01). ② Compared with the normal control group

the model control group's lung tissue and colon tissue showed serious inflammation; after drug treatment for 3 days and 7 days

Maxing Shigan Tang group showed obvious decrease in pathological damage of lung tissue and colon tissue. ③ Compared with the normal control group

MCP-1 protein expression in lung tissue of the model control group increased

with significant differences (P<0.01); after drug treatment for 3 days and 7 days

Maxing Shigan Tang group showed decrease in MCP-1 protein expression

with significant differences (P<0.01); there was a similar trend in the detection result of Western blot and immunohistochemistry. ④ Compared with the normal control group

MCP-1 protein expression in colon tissue of the model control group increased

with significant differences (P<0.05)

after drug treatment for 3 days and 7 days

Maxing Shigan Tang group showed no statistically significant difference in MCP-1 protein expression. ⑤ The lung index inhibiting effect of Maxing Shigan Tang in the influenza virus lung infection model had a positive correlation with the regulating effect in MCP-1 protein expression level of lung tissue and colon tissue. Conclusion:The influenza virus lung infection model has a serious colon tissue pathological injury

and the MCP-1 protein expression levels in lung tissue and colon tissue increased. Maxing Shigan Tang can play a role in anti-flu virus by regulating MCP-1 protein expression.

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