养阴润肠方对便秘小鼠结肠水通道蛋白3/9的影响

蒋峰; 周锦勇; 刘明浩; 朱萱萱; 朱长乐; 杨柏霖; 陈玉根

doi:10.13422/j.cnki.syfjx.2018050114

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养阴润肠方对便秘小鼠结肠水通道蛋白3/9的影响

药理 | 更新时间：2024-01-04

- 养阴润肠方对便秘小鼠结肠水通道蛋白3/9的影响
- Effect of Yangyin Runchang Decoction on Colonic Water Channel Protein 3 and Aquaporin 9 in Constipated Mice
- 中国实验方剂学杂志 2018年24卷第5期页码：114-120
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81373645）;江苏高校优势学科建设工程项目（CCI20140822）
- DOI：10.13422/j.cnki.syfjx.2018050114
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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蒋峰, 周锦勇, 刘明浩, 等. 养阴润肠方对便秘小鼠结肠水通道蛋白3/9的影响[J]. 中国实验方剂学杂志, 2018,24(5):114-120.

JIANG Feng, ZHOU Jin-yong, LIU Ming-hao, et al. Effect of Yangyin Runchang Decoction on Colonic Water Channel Protein 3 and Aquaporin 9 in Constipated Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 114-120.
蒋峰, 周锦勇, 刘明浩, 等. 养阴润肠方对便秘小鼠结肠水通道蛋白3/9的影响[J]. 中国实验方剂学杂志, 2018,24(5):114-120. DOI： 10.13422/j.cnki.syfjx.2018050114.

JIANG Feng, ZHOU Jin-yong, LIU Ming-hao, et al. Effect of Yangyin Runchang Decoction on Colonic Water Channel Protein 3 and Aquaporin 9 in Constipated Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 114-120. DOI： 10.13422/j.cnki.syfjx.2018050114.

摘要

目的：探讨养阴润肠方对便秘小鼠结肠水通道蛋白3（AQP3）和水通道蛋白9（AQP9）表达的影响。方法：50只雄性ICR小鼠随机分为正常组，模型组，养阴润肠方低、高剂量组，普卢卡必利组，每组10只，采用复方地芬诺酯20 mg ·kg-1连续灌胃15 d建立便秘模型，每周计算粪便含水量，用养阴润肠方低、高剂量组（29.6，59.2 g ·kg-1）干预15 d，取材结肠组织，进行免疫组化AQP3和AQP9定位和半定量表达，实时荧光定量聚合酶链式反应（Real-time PCR），蛋白免疫印迹法（Western blot）检测AQP3和AQP9基因和蛋白表达情况。结果：与正常组比较，模型组粪便含水量明显下降（P<0.01），AQP3表达增加（P<0.01），AQP3的mRNA和蛋白表达均增加（P<0.05），AQP9表达减少，AQP9的mRNA和蛋白表达亦均下降，但无统计学差异；与模型组比较，养阴润肠方低、高剂量组粪便含水量均增加（P<0.05），AQP3表达下降（P<0.05），AQP3的mRNA和蛋白表达均下降（P<0.05）；养阴润肠方低剂量组的AQP9表达增加（P<0.01），AQP9的mRNA和蛋白表达均升高（P<0.05），高剂量组表达有升高趋势，但无统计学差异。结论：养阴润肠方可以增加便秘小鼠粪便含水量，其机制可能与降低结肠组织中AQP3和增加AQP9的表达有关，并且大剂量作用反而不明显。

Abstract

Objective:To investigate the effect of Yangyin Runchang decoction on the expressions of aquaporin 3 (AQP3) and aquaporin 9 (AQP9) in constipated mice. Method:Fifty male ICR mice were randomly divided into normal group

model group

low-dose group

high-dose group and prucalopride group

with 10 mice in each group. The mice were gavaged with compound diphenoxylateat (20 mg·kg-1) for 15 days

then treated with Yangyin Runchang decoction at the doses of 29.6

59.2 g·kg-1 for 15 days. The colon tissues was collected

the locations and semi-quantified expressions of AQP3 and AQP9 were detected by immunohistochemistry

the mRNA and protein expressions of AQP3 and AQP9 were detected by Real-time PCR and Western blot. Result:Compared with the normal group

the fecal water content of the model group decreased significantly (P<0.01)

AQP3 expression increased (P<0.01)

the mRNA and protein expressions of AQP3 also increased (P<0.05)

AQP9 expression decreased

and the mRNA and protein expressions of AQP9 were also decreased

but with no significant difference. Compared with the model group

the fecal water content in treated groups increased (P<0.05)

AQP3 expression decreased (P<0.05)

and the mRNA and protein expressions of AQP3 were also decreased (P<0.05). The expression of AQP9 in the low-dose group increased (P<0.01)

and the mRNA and protein expressions of AQP9 increased (P<0.05)

while there was no significant difference in the high-dose group. Conclusion:Yangyin Runchang decoction can increase the water content of feces in constipated mice. Its mechanism may be correlated with the decreased expression of AQP3 and increased expression of AQP9 in colon tissues

but the effect of high dose is not obvious on the contrary.

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