Objective:To observe the effect of Yifei Zhuji prescription on cells activity

apoptosis

and the protein expression of epidermal growth factor receptor (EGFR) and G protein-coupled receptor 30 (GPR30) of human lung adenocarcinoma A549 cells

investigate its mechanism on inhibiting the activity of lung cancer cells and inducing apoptosis of lung cancer cells. Method:Human lung adenocarcinoma A549 cells were cultured to a logarithmic phase; 2

1.5 g·L-1 drug culture medium was added in Yifei Zhuji prescription high and low dose groups; 2.5 g·L-1 5-FU drug culture medium was added in blank group; RPMI 1640 culture medium containing 10% fetal bovine serum was added in solvent group. After treatment for 24

48 h

3-(4

5-dimethylthiazol-2-yl)-2

5-diphenyltetrazolium bromide (MTT) assay was used to detect the effect of Yifei Zhuji prescription on the activity of human lung adenocarcinoma A549 cells. Acridine orange/ethidium bromide (AO/EB) was used to observe the morphological changes of apoptosis of human lung adenocarcinoma A549 cells. Flow cytometry (FCM) was used to detect the apoptosis rate of A549 cells

and the morphological changes of A549 cells were observed by inverted microscope. In addition

Western blot was used to determine the apoptosis-related protein expression of EGFR and GPR30. Result:Compared with solvent group

both 1.5 and 2 g·L-1 Yifei Zhuji prescription could inhibit the activity of A549 cells(P<0.05)

and induce the apoptosis morphological changes of A549 cells; FCM results showed that A549 cells were induced to have late stage apoptosis after Yifei Zhuji prescription treatment for 24 h and 48 h(P<0.05). Western blot results showed that Yifei Zhuji prescription could down-regulate the apoptosis-related protein expression of EGFR and GPR30 to different degrees(P<0.05). Conclusion:Yifei Zhuji prescription could inhibit the activity of A549 cells

induce the apoptosis of A549 cells and induce the apoptosis morphological changes. The mechanism might be associated with down-regulating the protein expression of EGFR and GPR30.