黔产莪术油对直肠癌SW1463细胞株分泌Toll样受体及相关免疫因子的影响

许政旭; 朱诗国; 潘年松; 罗俊; 廖彬迅

doi:10.13422/j.cnki.syfjx.2018050137

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黔产莪术油对直肠癌SW1463细胞株分泌Toll样受体及相关免疫因子的影响

药理 | 更新时间：2024-01-04

- 黔产莪术油对直肠癌SW1463细胞株分泌Toll样受体及相关免疫因子的影响
- Effect of Guizhou Zedoary Oil on Toll Like Receptor and Related Immune Factors in SW1463 Cell Line
- 中国实验方剂学杂志 2018年24卷第5期页码：137-141
- 作者机构：
- 作者简介：
- 基金信息：
  
  贵州省科技计划项目（黔科合LG字[2011]006号）;贵州省社会发展项目（黔科合SY（2008）3027号）
- DOI：10.13422/j.cnki.syfjx.2018050137
  中图分类号： R285
- 纸质出版日期：2018
- 稿件说明：
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许政旭, 朱诗国, 潘年松, 等. 黔产莪术油对直肠癌SW1463细胞株分泌Toll样受体及相关免疫因子的影响[J]. 中国实验方剂学杂志, 2018,24(5):137-141.

XU Zheng-xu, ZHU Shi-guo, PAN Nian-song, et al. Effect of Guizhou Zedoary Oil on Toll Like Receptor and Related Immune Factors in SW1463 Cell Line[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 137-141.
许政旭, 朱诗国, 潘年松, 等. 黔产莪术油对直肠癌SW1463细胞株分泌Toll样受体及相关免疫因子的影响[J]. 中国实验方剂学杂志, 2018,24(5):137-141. DOI： 10.13422/j.cnki.syfjx.2018050137.

XU Zheng-xu, ZHU Shi-guo, PAN Nian-song, et al. Effect of Guizhou Zedoary Oil on Toll Like Receptor and Related Immune Factors in SW1463 Cell Line[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(5): 137-141. DOI： 10.13422/j.cnki.syfjx.2018050137.

摘要

目的：通过研究黔产莪术油对直肠癌SW1463细胞中死亡因子受体（Fas）的蛋白表达及分泌的免疫因子Toll样受体2（TLR2），Toll样受体4（TLR4），白细胞介素-10（IL-10），癌基因C-Raf转化生长因子-β1（TGF-β1）的影响，探讨黔产莪术油对肿瘤细胞的免疫效应。方法：将水蒸气蒸馏提取的黔产莪术油，配置成空白组，黔产莪术油80，120，160，200 mg ·L-1组，作用于直肠癌细胞SW1463细胞24 h后，采用免疫酶联免疫吸附测定（enzymeLinked immunosorbent assay，ELISA）检测不同质量浓度黔产莪术油对IL-10水平的影响，采用蛋白免疫印迹法（Western blot）检测Fas，TLR2，TLR4，C-Raf，TGF-β1蛋白表达对直肠癌细胞SW1463的增殖抑制作用。结果：ELISA检测发现，与空白组比较，莪术油组IL-10水平随着药物浓度的升高表达降低（F=23.11，P<0.01）。Western blot结果显示，与空白组比较，莪术油组随着药物浓度的升高，TLR2，TLR4，C-Raf蛋白表达显著降低（P<0.01）；Fas，TGF-β1蛋白表达明显降低（P<0.05）。结论：黔产莪术油抑制SW1463细胞的作用免疫作用机制可能与下调Fas/FasL通路有关，使得Toll样受体TLR2，TLR4蛋白表达下调，导致相关因子TGF-β1的表达下调，从而使得细胞凋亡，起到免疫增强作用。

Abstract

Objective:To Study the effect of Guizhou zedoary oil on death factor receptor (FAS) protein expression

Toll like receptor 2 (TLR2)

Toll like receptor 4 (TLR4) and interleukin-10 (IL-10)

C-Raf

transforming growth factor beta 1 (TGF-β1) in rectal cancer cells SW1463

and investigate the immune effect of Guizhou zedoary oil on tumor cells. Method:Guizhou zedoary oil extracted by steam distillation was divided into 80

120

160

200 mg·L-1 groups

and another blank group was also set up. After 24 h treatment on rectal cancer cell SW1463

enzyme linked immunosorbent assay (ELISA) was used to detect the different doses of Guizhou zedoary oil on the expression of IL-10; and Western blot method was used to detect Fas

TLR2

TLR4

C-Raf and TGF-β1 protein expression levels and investigate its inhibitory effect on rectal cancer cells SW1463 proliferation. Result:ELISA results showed that as compared with blank control group

IL-10 expression was down-regulated with the increase of concentration in Guizhou zedoary oil groups (F=23.11

P<0.01). Western blot results showed that the protein expression levels of TLR2

TLR4 and C-Raf were significantly decreased with the increase of drug concentration (P<0.01)

and the protein expression levels of Fas and TGF-β1 were also decreased (P<0.05). Conclusion:The immune mechanism of Guizhou zedoary oil in inhibiting SW1463 cells may be associated with down-regulation of Fas/FasL pathway

down-regulation of Toll like receptor TLR2 and TLR4 protein expression

leading to the down-reulation of relate factor TGF-β1

so that cell apoptosis is achieved to enhance the immune function.

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