Objective: To explore the correlation between the content of chemical components and effect index in the intestinal absorption fluid of Ephedrae Herba-Armeniacae Semen Amarum with different proportions

and clarify the material basis and mechanism of the medicine pair.Method: The U6(64) uniform design was used to arrange the proportions of this medicine pair

the major differential components in intestinal absorption fluid of this medicine pair and their contents were analyzed by UPLC-Q-TOF-MS/MS.Effect of intestinal absorption fluid containing drug on diastolic rate of tracheal ring in rats and the content of interleukin-2 (IL-2)

IL-4

tumor necrosis factor-α (TNF-α) in the rat tissue was investigated.The correlation between the effect index and the main component content was analyzed by multiple linear regression.Result: After being analyzed by the least squares regression

the significant difference components found by variable importance in the projection (VIP)>1.5 were norephedrine (X1)

pseudoephedrine (X2)

methyl ephedrine (X3) and amygdalin (X4).The medicine pair can obviously relieve the bronchial ring (Y1)

increase content of IL-2 (Y2)

decrease contents of IL-4 (Y3) and TNF-α (Y4) in the tissue.Taking contents of major differential components as independent variables (X)

effect index as dependent variables (Y)

regression equations were obtained as follows:Y1=-4 810.119X1+3 516.816X2-2 663.871X3-81.310X4+33.562(r=0.937)

Y2=4 203.354X1-2 567.853X2+1 445.790X3+47.460X4+237.192(r=0.996)

Y3=587.549X1-456.975X2+366.365X3+6.239X4+27.519(r=0.951)

Y4=-21 958.981X1-35 885.568X2+40 943.441X3+944.167X4+4 465.528(r=0.968).Conclusion: Main material bases of Ephedrae Herba-Armeniacae Semen Amarum on asthma are norephedrine

pseudoephedrine

methyl ephedrine and amygdalin

its antiasthmatic mechanism is related to bidirectional regulating airway function of each effect component

changes in IL-4

IL-2 and TNF-α content and so on.