Objective: To develop a HPLC method for simultaneous determination of seven components (chiratin

loganin

loganic acid

chlorogenic acid

asperosaponin Ⅵ

dipsanoside A and dipsanoside B) in Dipsaci Radix

so as to provide scientific basis for the comprehensive quality evaluation and control of Dipsaci Radix.Method: Samples were analyzed on a Venusil HILIC column(4.6 mm×250 mm

5 μm)

and eluted with the mobile phase of acetonitrile(A) and 0.1% phosphoric acid(B) with a linear gradient mode(0-20 min

95% A;20-40 min

95%-87% A;40-64 min

87%-84% A;64-65 min

84%-78% A;65-80 min

78%-77% A)

the flow rate of the mobile phase was 1.0 mL ·min-1 with the column temperature of 30℃

and the detection wavelengths were set at 235 nm and 212 nm

respectively.Result: These seven components were well separated and showed good linearity(r ≥ 0.999 9) within the concentration ranges tested

the mean recoveries were 99.11%-102.24% with the RSD between 0.2%-1.5%.The content of asperosaponin Ⅵ in 13 samples conformed to standard in the 2015 edition of Chinese Pharmacopoeia

but the content of the seven components in each sample showed a great differences.Conclusion: The method is simple

accurate and repeatable

it can be used for the determination of multiple components in Dipsaci Radix.