Objective: To establish a rapid analysis method for determining the contents of rehmaionoside A

rehmaionoside D and leonuride in Rehmanniae Radix.Method: Near-infrared spectra of six varieties of Rehmanniae Radix (108 samples) were obtained by near infrared diffuse reflectance spectroscopy (NIRS) technique. By using high performance liquid chromatography (HPLC) method

the contents of rehmannioside A

rehmaionoside D and leonuride were detected and their values were correlated with the spectra information by using TQ Software. Then the quantitative models of rehmaionoside A

rehmaionoside D and leonuride in Rehmanniae Radix were built by partial least squares (PLS).Result: The correlation coefficients (R2) of the models was 0.924 67

0.934 96 and 0.951 54; the root mean square error of calibration(RMSEC) was 0.016 6

0.015 9 and 0.022 8; the root mean square error of prediction (RMSEP) was 0.017 00

0.007 86 and 0.012 50; the root mean square error of cross validation (RMSECV) was 0.032 13

0.030 36 and 0.069 22; and the performance index (PI) was 92.5

82.7 and 83.1 respectively. Besides

paired sample t-test showed that the P value of NIR and HPLC was 0.422

0.549 and 0.131

all higher than 0.05

indicating that there was no significant difference between the two methods.Conclusion: The NIR method is accurate

rapid

and green. It can be used for quantitative analysis of rehmaionoside A

rehmaionoside D and leonuride in Rehmanniae Radix.