续骨丹对胫骨骨不连大鼠VEGF，TGF-表达的影响

李月玮; 周围; 郭佳宝; 宋亚芳; 王旭东

doi:10.13422/j.cnki.syfjx.20180628

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续骨丹对胫骨骨不连大鼠VEGF，TGF-表达的影响

药理 | 更新时间：2024-01-04

- 续骨丹对胫骨骨不连大鼠VEGF，TGF-表达的影响
- Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia
- 中国实验方剂学杂志 2018年24卷第8期页码：91-96
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医药管理局项目[ZYYS-2013-（0003）-2];国家社科基金重大项目（12ZD114）
- DOI：10.13422/j.cnki.syfjx.20180628
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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李月玮, 周围, 郭佳宝, 等. 续骨丹对胫骨骨不连大鼠VEGF，TGF-表达的影响[J]. 中国实验方剂学杂志, 2018,24(8):91-96.

LI Yue-wei, ZHOU Wei, GUO Jia-bao, et al. Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 91-96.
李月玮, 周围, 郭佳宝, 等. 续骨丹对胫骨骨不连大鼠VEGF，TGF-表达的影响[J]. 中国实验方剂学杂志, 2018,24(8):91-96. DOI： 10.13422/j.cnki.syfjx.20180628.

LI Yue-wei, ZHOU Wei, GUO Jia-bao, et al. Effect of Xugudan on Expression of VEGF and TGF- in Rats with Nonunion of Tibia[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 91-96. DOI： 10.13422/j.cnki.syfjx.20180628.

摘要

目的：研究续骨丹外敷疗法对大鼠胫骨不连模型血管内皮生长因子（vascular endothelial growth factor，VEGF）及转化生长因子-β（transforming growth factor-β，TGF-β）表达的影响。方法：选取10只SD大鼠为空白组，成模50只SD大鼠随机分为模型组，续骨丹高、中、低剂量组（150，75，25 mg·kg-1），经皮电刺激神经组（transcutaneous electrical nerve stimulation，TENS）。分别在干预后4，8周对大鼠行X射线检测、测量骨间距；苏木素-伊红（HE）染色观察组织形态变化；实时荧光定量PCR（Real-time PCR）和酶联免疫吸附法（enzyme linked immunosorbent assay，ELISA）分别检测骨损伤区和血清中VEGF，TGF-β变化。结果：与模型组比较，续骨丹高、中、低剂量组，TENS组骨间距和VEGF，TGF-β表达显著升高（P<0.01）；与TENS组比较，续骨丹高剂量组骨间距和VEGF，TGF-β表达显著升高（P<0.01），续骨丹中剂量组差异不显著，续骨丹低剂量组显著降低（P<0.05，P<0.01）。结论：续骨丹可以通过调节VEGF，TGF-β的表达，改善局部血流供应，诱导血管重建和骨重建，促进骨不连愈合。

Abstract

Objective: To observe the effect ofexternal application of Xugudan on expression of vascular endothelial growth factor(VEGF) and transforming growth factor-β (TGF-β) in rat model of tibial nonunion. Method: A total of 10 SD rats were selected as blank group

and 50 modeled SD rats were randomly divided into model group

high

middle and low dose Xugudan(150

25 mg · kg-1) groups

and transcutaneous electrical nerve stimulation (TENS) group. In 4 weeks

and 8 weeks after the intervention

X-ray detection and measurement of bone spacing were conducted; hematoxylin eosin (HE) staining was used to observe the morphological changes. Real-time PCR and enzyme linked immunosorbent assay (ELISA) were used todetect VEGF

and TGF-β changes in bone injury and serum. Result: As compared with the model group

bone spacing

VEGF and TGF-β expression levels were significantly increased in Xugudan high

middle and low dose groups as well as TENS group(P<0.01). As compared with TENS group

bone spacing

VEGF and TGF-β expression levels were significantly increased in Xugudan high dose group (P<0.01)

butthe difference was not significant from Xugudan middle dose group

and the levels were significantly decreased in Xugudan low dose group (P<0.01

P<0.05). Conclusion: Xugudan can improve the local blood supply by regulating the expression of VEGF and TGF-β

induce vascular remodeling and bone remodeling

and promote the nonunion healing.

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