金蝉花多糖对-GlaN致小鼠急性肝损伤的保护作用及机制

温萍; 陈盛铎; 郑兵; 梁海莉; 汪婷; 白丽英; 陶君秀; 张弦; 王家瑞

doi:10.13422/j.cnki.syfjx.20180630

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金蝉花多糖对-GlaN致小鼠急性肝损伤的保护作用及机制

药理 | 更新时间：2024-01-04

- 金蝉花多糖对-GlaN致小鼠急性肝损伤的保护作用及机制
- Protective Effect and Mechanism of Polysaccharides from on Acute Liver Injury Induced by -GlaN in Mice
- 中国实验方剂学杂志 2018年24卷第6期页码：108-113
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家中医临床研究基地业务建设科研专项（JDZX2012059）
- DOI：10.13422/j.cnki.syfjx.20180630
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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温萍, 陈盛铎, 郑兵, 等. 金蝉花多糖对-GlaN致小鼠急性肝损伤的保护作用及机制[J]. 中国实验方剂学杂志, 2018,24(6):108-113.

WEN Ping, CHEN Sheng-duo, ZHENG Bing, et al. Protective Effect and Mechanism of Polysaccharides from on Acute Liver Injury Induced by -GlaN in Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 108-113.
温萍, 陈盛铎, 郑兵, 等. 金蝉花多糖对-GlaN致小鼠急性肝损伤的保护作用及机制[J]. 中国实验方剂学杂志, 2018,24(6):108-113. DOI： 10.13422/j.cnki.syfjx.20180630.

WEN Ping, CHEN Sheng-duo, ZHENG Bing, et al. Protective Effect and Mechanism of Polysaccharides from on Acute Liver Injury Induced by -GlaN in Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 108-113. DOI： 10.13422/j.cnki.syfjx.20180630.

摘要

目的：观察金蝉花多糖对D-半乳糖胺（D-galactosamine，D-GlaN）致小鼠急性肝损伤的保护作用，并初步探讨其作用机制。方法： 75只雄性昆明小鼠根据数字表法随机分为5组，每组15只，正常组，腹腔注射生理盐水；模型组，腹腔注射D-GlaN；金蝉花多糖低、中、高剂量组，腹腔注射D-GlaN的同时予以金蝉花多糖溶液（0.5，1.0，2.0 g ·kg-1）灌胃治疗。治疗12 d后检测各组肝组织病理评分、肝匀浆指标[超氧化物歧化物（superoxide dismutase，SOD），丙二醛（malondialdehyde，MDA），谷胱甘肽过氧化物酶（glutathione peroxidase，GSH-Px），一氧化氮（nitric oxide，NO）]，血清学指标[天门冬氨酸氨基转移酶（aspartate transaminase，AST），丙氨酸氨基转移酶（alanine transaminase，ALT），碱性磷酸酶（alkaline phosphatase，ALP），胆碱酯酶（cholinesterase，CHE）]，采用免疫组化法检测各组肝组织核转录因子-κB （nuclear transcription factor-κB，NF-κB），肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）表达水平。结果：模型组肝组织病理评分，MDA，NO，AST，ALT，ALP，NF-κB，TNF-α水平均明显高于正常组（P<0.05），而SOD，GSH-Px，CHE水平明显低于正常组（P<0.05）；金蝉花多糖低、中、高剂量组肝组织病理评分均明显低于模型组（P<0.05），且随治疗剂量升高，肝组织病理评分呈明显降低趋势（P<0.05）；金蝉花多糖低、中、高剂量组MDA，NO，AST，ALT，ALP，NF-κB，TNF-α水平均明显低于模型组（P<0.05），而SOD，GSH-Px，CHE水平均明显高于模型组（P<0.05），且随治疗剂量升高，MDA，NO，AST，ALT，ALP，NF-κB，TNF-α水平明显降低（P<0.05），而SOD，GSH-Px，CHE水平明显升高（P<0.05）。结论：金蝉花多糖对D-GlaN致小鼠急性肝损伤具有明显的保护作用，且呈剂量依赖性，其作用机制可能为抑制NF-κB炎性反应信号通路有关。

Abstract

Objective: To observe the protective effect of polysaccharides from Cordyceps cicadae on acute liver injury induced by D-galactosamine (D-GlaN) in mice

and to explore its mechanism.Method: Seventy-five male Kunming mice were randomly divided into 5 groups (n=15 for each group). The miceinnormal group were injected with saline solution intraperitoneally while the mice in model group were intraperitoneally injected with D-GlaN. The mice in polysaccharides from C. cicadae low dose

middle dose and high dose groups were intraperitoneally injected with D-GlaN and lavage treatment with polysaccharides from C. cicadae solution (0.5

1.0

2.0 g · kg-1 respectively). After 12 days of treatment

the mouse liver homogenate indexes[superoxide dismutase (SOD)

malondialdehyde(MDA)

glutathione peroxidase(GSH-Px)

nitric oxide(NO)]

and serum markers[aspartate transaminase(AST)

alanine transaminase(ALT)

alkaline phosphatase(ALP)

cholinesterase(CHE)]were detected together withthe liver tissue pathological scores in each groups. The expression levels of nuclear transcription factor-κB (NF-κB) and tumor necrosis factor-α (TNF-α) were detected in the liver tissues by immunohistochemistry.Result: The liver histopathological scores

MDA

AST

ALT

ALP

NF-κB

and TNF-α in model group were significantly higher than those in normal group (P<0.05)

while the levels of SOD

GSH-Px and CHE were significantly lower than those in normal group(P<0.05). The liver histopathological scores in the polysaccharides from C. cicadae low

middle and high dose groups were significantly lower than those in model group (P<0.05)

moreover

the liver histopathological scores tended to decrease with the increase of therapeutic dose (P<0.05); the levels of MDA

AST

ALT

ALP

NF-κB

and TNF-α in the low

middle and high dose groups were significantly lower than those in model group (P<0.05)

while the levels of SOD

GSH-Px and CHE were significantly higher than those in the model group (P<0.05)

and as the therapeutic dose increased

the levels of MDA

AST

ALT

ALP

NF-κB and TNF-α were significantly decreased (P<0.05)

while SOD

GSH-Px

CHE levels were significantly increased (P<0.05).Conclusion: Polysaccharides from C. cicadae may have protective effect on D-GlaN-induced acute liver injury in a dose-dependent manner

and its mechanismmay be related to the inhibition of NF-κB inflammatory signaling pathway.

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