金铁锁及其总皂苷体内抗炎作用分析

邓祎; 钱子刚; 刘武林; 丁雄; 陈海丰

doi:10.13422/j.cnki.syfjx.20180633

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金铁锁及其总皂苷体内抗炎作用分析

药理 | 更新时间：2024-01-04

- 金铁锁及其总皂苷体内抗炎作用分析
- Anti-inflammatory Activity of Psammosilenes Radix and Total Saponins
- 中国实验方剂学杂志 2018年24卷第7期页码：165-170
- 作者机构：
- 作者简介：
- 基金信息：
  
  云南省应用基础研究计划项目（2014FZ071）
- DOI：10.13422/j.cnki.syfjx.20180633
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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邓祎, 钱子刚, 刘武林, 等. 金铁锁及其总皂苷体内抗炎作用分析[J]. 中国实验方剂学杂志, 2018,24(7):165-170.

DENG Yi, QIAN Zi-gang, LIU Wu-lin, et al. Anti-inflammatory Activity of Psammosilenes Radix and Total Saponins [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 165-170.
邓祎, 钱子刚, 刘武林, 等. 金铁锁及其总皂苷体内抗炎作用分析[J]. 中国实验方剂学杂志, 2018,24(7):165-170. DOI： 10.13422/j.cnki.syfjx.20180633.

DENG Yi, QIAN Zi-gang, LIU Wu-lin, et al. Anti-inflammatory Activity of Psammosilenes Radix and Total Saponins [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 165-170. DOI： 10.13422/j.cnki.syfjx.20180633.

摘要

目的：探讨金铁锁（PR）及其总皂苷（PRTS）的抗炎镇痛作用，并探讨其抗炎机制。方法：实验动物随机分为空白组（大鼠），模型组（大鼠、小鼠），阳性药组（小鼠：吲哚美辛，元胡止痛胶囊0.585 g ·kg-1），金铁锁75%乙醇提取物（PR）高、中、低剂量组（小鼠：0.651 1，0.217，0.072 35 g ·kg-1，大鼠：0.450 8，0.150 3，0.050 08 g ·kg-1），金铁锁总皂苷（PRTS）高、低剂量组（小鼠：0.233 4，0.025 94 g ·kg-1，大鼠：0.161 6，0.017 96 g ·kg-1）。采用醋酸诱导扭体实验和热板实验来评价镇痛作用；采用二甲苯诱导小鼠耳肿胀实验，脂多糖（LPS）诱导大鼠腹膜炎来评价抗炎作用，通过苏木素-伊红（HE）染色观察大鼠腹膜组织的病理变化，酶联免疫吸附法（ELISA）测定血清中的炎症因子白细胞介素（IL）-6，IL-10。探寻潜在机制，通过大鼠肠系膜组织免疫组化观察肿瘤坏死因子（TNF）-α，IL-1β，核转录因子-κB（NF-κB）p65的表达。结果：与空白组比较，PR及PRTS各剂量组减轻小鼠耳肿胀（P<0.01），减少小鼠化学致痛扭体次数（P<0.05，P<0.01）；PRTS高剂量组能提高小鼠痛阈值（P<0.05，P<0.01）；与模型组比较，PR及PRTS各剂量组对急性腹膜炎大鼠腹膜组织病变有所改善，能降低炎症因子IL-6含量（P<0.01），同时降低TNF-α，IL-1β，NF-κB p65在肠系膜组织的表达，PR高剂量及PRTS高剂量组能降低IL-10含量（P<0.05）。结论：金铁锁及总皂苷具有一定的镇痛抗炎作用，其机制可能与下调炎症因子的释放与表达，抑制NF-κB信号通道有关。

Abstract

Objective: To investigate the anti-inflammatory and analgesic effects of Psammosilenes Radix (PR) and PR total saponins (PRTS)

and exploreits possible mechanism of anti-inflammatory. Method: The animals were randomly divided into blank control group

model control group

positive drug group (mice:indomethacin

Yuanhu Zhitong capsule 0.585 g · kg-1)

PR high

medium and low dosegroups (mice:0.651 1

0.217

0.072 35 g · kg-1

rats:0.450 8

0.150 3

0.050 08 g · kg-1)

PRTS high and low dose groups (mice:0.233 4

0.025 94 g · kg-1

rats:0.161 6

0.017 96 g · kg-1). The analgesic effect was evaluated by acetic acid-induced writhing experiments and hot plate experiments. Xylene-induced mouse ear swelling test and lipopolysaccharide (LPS) induced acute peritonitis in rats were used to evaluate itsanti-inflammatory effect; the pathological changes of peritoneal tissue were observedby hematoxylin-eosin (HE) staining; cell factor of interleukin(IL)-6

IL-10 were detected with enzyme-linked immunosorbent assay(ELISA). The expression levels of tumor necrosis factor (TNF)-α

IL-1β and nuclear factor-kappaB(NF-κB) p65 were detected by immunohistochemistry in rat mesenteric tissues. Result: As compared with the blank control group

PR and PRTS treatment groups could inhibit the mice ear swelling (P<0.01)

and obviously decrease writhing times (P<0.05

P<0.01); PRTS high dose group could remarkably improve the pain threshold of hot-plate test (P<0.01). As compared with the model group

PR and PRTS treatment groups could improve the peritoneal histopathology of acute peritonitis rats and decrease the content of IL-6 (P<0.01); the expression levels of TNF-α

IL-1β and NF-κB p65 in the mesenteric tissues were significantly decreased; and the high dose of PR and PRTS could significantly decrease the levels of IL-10 (P<0.05). Conclusion: PR and PRTS have certain analgesic and anti-inflammatory effects

and its mechanism may be related to down-regulating the expression of inflammatory factors and inhibiting the NF-κB signaling pathway.

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