健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及Caspase-3,Bcl-xL,Bax蛋白表达的影响

简小兰; 何兰; 李勇敏; 吕元; 谭小宁; 罗吉; 罗燕; 蒋益兰

doi:10.13422/j.cnki.syfjx.20180684

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健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及Caspase-3,Bcl-xL,Bax蛋白表达的影响

健脾消癌方抑制结直肠癌转移分子机制研究专题 | 更新时间：2024-01-04

- 健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及Caspase-3,Bcl-xL,Bax蛋白表达的影响
- Effect of Jianpi Xiaoai Prescription and Its Decomposed Recipes on Body Weight, Tumors, Metastasis Rate and Expressions of Caspase-3, Bcl-xL, Bax in Colorectal Cancer Model Nude Mice
- 中国实验方剂学杂志 2018年24卷第6期页码：156-160
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81373602，81774287）;中央引导地方科技发展专项（2017CT5029）
- DOI：10.13422/j.cnki.syfjx.20180684
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
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简小兰, 何兰, 李勇敏, 等. 健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及Caspase-3,Bcl-xL,Bax蛋白表达的影响[J]. 中国实验方剂学杂志, 2018,24(6):156-160.

JIAN Xiao-lan, HE Lan, LI Yong-min, et al. Effect of Jianpi Xiaoai Prescription and Its Decomposed Recipes on Body Weight, Tumors, Metastasis Rate and Expressions of Caspase-3, Bcl-xL, Bax in Colorectal Cancer Model Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 156-160.
简小兰, 何兰, 李勇敏, 等. 健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及Caspase-3,Bcl-xL,Bax蛋白表达的影响[J]. 中国实验方剂学杂志, 2018,24(6):156-160. DOI： 10.13422/j.cnki.syfjx.20180684.

JIAN Xiao-lan, HE Lan, LI Yong-min, et al. Effect of Jianpi Xiaoai Prescription and Its Decomposed Recipes on Body Weight, Tumors, Metastasis Rate and Expressions of Caspase-3, Bcl-xL, Bax in Colorectal Cancer Model Nude Mice[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 156-160. DOI： 10.13422/j.cnki.syfjx.20180684.

摘要

目的：观察健脾消癌方及其拆方对肠癌模型裸鼠体质量、瘤体、转移率及半胱氨酸天冬氨酸蛋白酶-3（Caspase-3），抗凋亡蛋白B细胞淋巴瘤/白血病-xL （B-cell lymphoma/leukemia-xL，Bcl-xL）和Bcl-2相关X蛋白（Bcl-2 associated X protein，Bax）表达的影响。方法：建立裸鼠肠癌原位瘤模型，分为模型组，健脾益气组（15 g · kg-1），化瘀解毒组（15 g · kg-1），健脾消癌方组（全方组，15 g · kg-1），5-氟尿嘧啶组（5-Fu，20 mg · kg-1），共5组。分别予以相应药物干预4周，观察裸鼠体重、瘤体抑制率、转移率，蛋白免疫印迹法（Western blot）检测Caspase-3，Bcl-xL，Bax蛋白表达。结果：健脾益气组、全方组体重下降低于模型组（P<0.05），化瘀解毒组体重下降高于模型组（P<0.05）；健脾益气组对瘤体的抑制与模型组比较无统计学差异，与空白组比较，化瘀解毒组、全方组对瘤体均有较好抑制作用（P<0.05）；各组均出现不同程度地转移，其中模型组、健脾益气组总体转移率达90%，化瘀解毒组、全方组转移率分别为70%，60%，低于模型组（P<0.05）；与模型组比较，化瘀解毒组及全方组能够上调Bax蛋白表达，下调Caspase-3，Bcl-xL蛋白表达（P<0.01）。结论：健脾消癌方中健脾益气组优势在于稳定体重，化瘀解毒组优势在于抑制肿瘤，抑制转移；全方组能够稳定体质量，抑制肿瘤，抑制转移，全方配伍疗效最优，其抗肿瘤机制可能是上调凋亡相关蛋白Bax及下调Caspase-3，Bcl-xL，诱导肿瘤细胞凋亡。

Abstract

Objective: To observe the effect of Jianpi Xiaoai prescription and its decomposed recipes on the body weight

tumors

metastasis rate and expressions of Caspase-3

B-cell lymphoma/leukemia-xL (Bcl-xL) and Bcl-2 associated X protein (Bax) in colorectal cancer model nude mice.Method: The colorectal cancer model was established

and divided into control group

the Jianpi Yiqi group (15 g · kg-1)

the Huayu Jiedu group (15 g · kg-1)

the Jianpi Xiaoai prescription group (omni-recipe group

15 g · kg-1) and the 5-fluorouracil group (5-Fu

20 mg · kg-1). The body weight

tumor inhibition rate and metastasis rate were observed

and the protein expressions of Caspase-3

Bcl-xL

and Bax of nude mice were detected by Western blot after the corresponding drug intervention for 4 weeks.Result: The weight loss of the Jianpi Yiqi group and the omni-recipe group was less than that of control group (P<0.05)

Huayu Jiedu group' weight loss was higher than that of control group (P<0.05). The Jianpi Yiqi group had no statistical differences from control group in tumor inhibition rate; the Huayu Jiedu group and the omni-recipe group had a better tumor inhibition rate (P<0.05). All of the groups had different degrees of metastasis. Specifically

the control group and the Jianpi Yiqi group had the overall metastasis rate of 90%

and the Huayu Jiedu group and the omni-recipe group had the metastasis rate of 70% and 60%

respectively

both of which were lower than that of control group (P<0.05). Compared with control group

the Huayu Jiedu group and the omni-recipe group showed increase in the protein expression of Bax

and decrease in the protein expressions of Caspase-3

Bcl-xL (P<0.01).Conclusion: The Jianpi Yiqi group shows a stable weight

while the Huayu Jiedu group shows inhibitions in tumor and metastasis. The omni-recipe group shows a stable weight and inhibitions in tumor and metastasis

with the best effect. The antitumor mechanism of Jianpi Xiaoai prescription may be correlated with the up-regulation in expressions of apoptosis-associated proteins Bax and the down-regulation in Caspase-3

Bcl-xL induced apoptosis of tumor cells.

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