结直肠癌转移模型裸鼠血管因子变化及健脾消癌方干预作用

李勇敏; 谭小宁; 徐琳本; 吕元; 奚剑敏; 蒋益兰

doi:10.13422/j.cnki.syfjx.20180686

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结直肠癌转移模型裸鼠血管因子变化及健脾消癌方干预作用

健脾消癌方抑制结直肠癌转移分子机制研究专题 | 更新时间：2024-01-04

- 结直肠癌转移模型裸鼠血管因子变化及健脾消癌方干预作用
- Changes of Blood Vessel Factors in Nude Mice Model with Colorectal Cancer Metastasis and Invention Effect of Jianpi Xiaoai Prescription
- 中国实验方剂学杂志 2018年24卷第6期页码：167-171
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金面上项目（81373602）;湖南省科技计划科研条件创新专项（2013TT1004）;湖南省自然科学基金项目（14J4067）
- DOI：10.13422/j.cnki.syfjx.20180686
  中图分类号： R-332;R285.5
- 纸质出版日期：2018
- 稿件说明：
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李勇敏, 谭小宁, 徐琳本, 等. 结直肠癌转移模型裸鼠血管因子变化及健脾消癌方干预作用[J]. 中国实验方剂学杂志, 2018,24(6):167-171.

LI Yong-min, TAN Xiao-ning, XU Lin-ben, et al. Changes of Blood Vessel Factors in Nude Mice Model with Colorectal Cancer Metastasis and Invention Effect of Jianpi Xiaoai Prescription[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 167-171.
李勇敏, 谭小宁, 徐琳本, 等. 结直肠癌转移模型裸鼠血管因子变化及健脾消癌方干预作用[J]. 中国实验方剂学杂志, 2018,24(6):167-171. DOI： 10.13422/j.cnki.syfjx.20180686.

LI Yong-min, TAN Xiao-ning, XU Lin-ben, et al. Changes of Blood Vessel Factors in Nude Mice Model with Colorectal Cancer Metastasis and Invention Effect of Jianpi Xiaoai Prescription[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(6): 167-171. DOI： 10.13422/j.cnki.syfjx.20180686.

摘要

目的：探讨结直肠癌裸鼠转移模型组织中血管内皮生长因子（VEGF），内皮抑素（ES），血管生成素（ANG），血管抑素（AS），基质金属蛋白酶-9（MMP-9），组织金属蛋白酶抑制物（TIMP）变化及健脾消癌方干预的机制。方法：在BALB/C-nu裸鼠盲肠内注射HCT116细胞50 μL，造裸鼠结直肠癌转移模型。将模型裸鼠分为模型组、健脾消癌方组（40 g · kg-1），另设空白组。所有动物灌胃给予相应药物4周后，蛋白免疫印迹法（Western blot）测定裸鼠结肠、肝、肺及盲肠肿瘤组织中相关血管因子蛋白表达。结果： HCT116结直肠癌模型裸鼠的结肠、肝、肺与肿瘤组织的VEGF，MMP-9，TIMP明显升高（P<0.05，P<0.01）；ANG在结肠、肝与肿瘤组织也明显升高（P<0.05，P<0.01）；ES在结肠、肺与肿瘤组织显著降低，AS在肝、肺及肿瘤组织显著降低（P<0.05）。健脾消癌方组的结肠MMP-9，肿瘤TIMP，肝组织ANG，MMP-9及肺组织VEGF明显降低，肺组织AS明显升高（P<0.05）。结论： HCT116结直肠癌模型裸鼠VEGF，ANG，MMP-9在结肠、肝、肺及肿瘤组织中蛋白表达升高，ES，AS降低，TIMP升高；健脾消癌方能比较全面地改善机体抗肿瘤血管生成的能力。

Abstract

Objective: To investigate the changes in vascular endothelial growth factor (VEGF)

endostatin (ES)

angiogenesis factors (ANG)

angiostatin (AS)

matrix metalloproteinase-9 (MMP-9) and tissue metalloproteinase inhibitor (TIMP) in tissues of nude mice with rectal cancer metastasis model

and the intervention mechanism of Jianpi Xiaoai prescription.Method: HCT116 cells were injected into the BALB/C-nu nude mice

so as to establish the model of colorectal cancer metastasis. The model nude mice were divided into model group and Jianpi Xiaoai prescription group; and control group was also set up. After 4 weeks of administration

the expression of vascular factor in colon

liver

lung and tumor tissues of nude mice was measured by Western blot.Result: VEGF

MMP-9 and TIMP-1 of colon

liver

lung and tumor tissues in HCT116 rectal cancer model nude mice increased significantly (P<0.05

P<0.01); ANG in colon

liver and tumor tissues was significantly increased (P<0.05

P<0.01); ES was significantly lower in colon

lung and tumor tissues

while as significantly reduced in liver

lung and tumor tissues (P<0.05). Colon MMP-9

tumor TIMP

liver tissue ANG

MMP-9 and lung tissue VEGF were significantly decreased in Jianpi Xiaoai prescription group

whereas AS was significantly increased (P<0.05) in lung tissues.Conclusion: The expressions of VEGF

ANG and MMP-9 of colon

liver

lung and tumor tissues in HCT116 colorectal cancer model nude mice increase

while ES

AS reduce

and TIMP rose. Jianpi Xiaoai prescription can comprehensively improve the anti-tumor angiogenesis capacity.

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