Objective: Distillation method was employed to determine content of Cyperi Rhizoma stir-fried with vinegar

which had features of wasting time

complex operation

without specific effective component content index in the quality standard.In order to solve these problems

this study was to explore the method for determination of specific active ingredients in Cyperi Rhizoma stir-fried with vinegar by advanced detection technology

combined with near-infrared spectroscopy

to establish a rapid and accurate quantitative analysis model. Method: HPLC was adopted to determine the content of α-cyperone with mobile phase of methanol-0.1% phosphoric acid for gradient elution and detection wavelength at 252 nm.The near infrared spectra of Cyperi Rhizoma stir-fried with vinegar were collected by near-infrared spectrometer.Taking HPLC measured value as a reference

partial least squares(PLS) was used to establish the quantitative model of α-cyperone. Result: The quantitative model of α-cyperone was established

the correlation coefficient of calibration (R2)

root-mean-square error of calibration(RMSEC) and root-mean-square error of prediction(RMSEP) of the calibration model were 0.987 5

0.006 2 and 0.007 2;the root-mean-square error of cross-validation(RMSECV) of the calibration model was 0.020 4

the average recovery was 101.33%. Conclusion: The method has fast detection speed and accurate prediction results

it can be applied to quickly determine the content of α-cyperone in Cyperi Rhizoma stir-fried with vinegar.