Objective: Desiccative ripe fruits of Foeniculum vulgare

Cuminum cyminum

Anethum graveolens

Carum carvi

Cnidium monnieri and Saposhnikovia divaricata were used as the research objects

these fruits had similar appearance and they are easy to be confused

which will give rise to security risks.To develop a high resolution melting curve(HRM) analysis method in a fast and easy way targeted on the problem of identification of these medicinal materials.Method: Detection method and melting curve model was established for identification based on internal transcribed spacer 2(ITS2) region of the four spices and their common adulterants of C. monnieri fruit and S. divaricata fruit

there were 23 reference samples.Then the four spices (including 17 market samples) were collected form spice market

and they were identified if the species was compatible with the label by HRM.Result: The melting curves of the four spices and their adulterants had clear diversity.The two of the 17 market samples

N1 and N3

were potentially mislabeled

and their sequencing results showed that both were Cicuta virosa.Conclusion: HRM technology can realize the visual identification of common Umbelliferae spices and their adulterants

and it can be used as a powerful supplement of DNA barcoding technology.