Objective: To investigate the influence of coumarins from Angelicae Dahuricae Radix

such as imperatorin

isoimperatorin and oxypeucedanin

on intestinal transport and pharmacokinetics of vincristine sulfate in rats.Method: MDCK-MDR1 cell model was used to investigate the effect of imperatorin

isoimperatorin and oxypeucedanin on intestinal transport of vincristine sulfate.The drug plasma concentration was determined by LC-MS/MS at different time

and the effect of coumarins from Angelicae Dahuricae Radix on the pharmacokinetics of vincristine sulfate was investigated.Result: The apparent permeability coefficient (Papp) of vincristine sulfate compatibility of 10.80

5.42

1.35 mg · L-1 of imperatorin were (0.287 2±0.026 6)×10-6

(0.351 5±0.041 6)×10-6

(0.294 9±0.006 8)×10-6 cm · s-1

they had significant difference (P<0.05) when compared with Papp=(0.227 2±0.014 8)×10-6 cm · s-1 of vincristine sulfate.AUC0-t of vincristine sulfate compatibility of imperatorin

isoimperatorin and oxypeucedanin were (343.50±10.29)

(348.70±27.99)

(235.70±17.56) μg · h · L-1

their Cmax were (81.87±8.517)

(83.01±9.276)

(60.44±6.679) μg · L-1;there were significant difference (P<0.01) when compared with the control group of vincristine sulfate.Conclusion: Coumarins from Angelicae Dahuricae Radix can promote the transport absorption of vincristine sulfate and improve its bioavailability.