XIONG Hui, TU Chu-yue, JIANG Hai-qin, et al. Chemical Constituents from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 49-53.
XIONG Hui, TU Chu-yue, JIANG Hai-qin, et al. Chemical Constituents from Stems of [J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 49-53. DOI: 10.13422/j.cnki.syfjx.20180713.
Objective: The stems of Entada phaseoloides have been used for the treatment of rheumatoid arthritis and traumatic injuries. In order to explore its bioactive constituents
70% ethanol extract was studied systemically in chemistry. Method: The dried and powdered stems (9 kg) of E. phaseoloides were extracted for tree times with 70% ethanol at room temperature (3×24 h). The combined ethanol extracts were extracted consecutively with petroleum ether
EtOAc and n-BuOH. The components of n-BuOH extract were isolated and purified by normal and reverse phase silica gels
Sephadex LH-20 and MCI column chromatography and semi-preparative HPLC. And their structures were confirmed based on the physicochemical properties and spectral analyses. Result: Ten compounds were isolated from the n-BuOH fraction of 70% ethanol extract from stems of E. phaseoloides
which were identified as epigallocatechin (1)
uridine (2)
gallocatechin (3)
catechin (4)
epicatechin (5)
procyanidin B3 (6)
procyanidin B1 (7)
eriocitrin (8)
(-)-aromadendrin-3-O-β-D-glucopyranoside (9) and naringin dihydrochalcone (10). Conclusion: Compounds 2 and 6-10 were isolated from this plant for the first time.