Objective: To establish an analysis method of Shuanghuanglian preparations for determination of multiple components by HPLC chromatographic

in order to assist the peak identification

and detect the prediction accuracy and column suitability. Method: The retention time of the nine components in Shuanghuanglian preparations were determined on 28 C18 columns of different brands and types. The standard retention time (SRT) was calculated. Both chlorogenic acid (peak 1) and wogonin (peak 9) were taken as the linear calibration standards to forecast the retention time of the other seven components. Other 5 C18 columns of different brands and types were used for validation. In comparison of liner calibration with the two reference substances

baicalin was used as the reference substance for the determination of relative correction factors of other eight components. Result: Liner calibration with two reference substances is more accurate for predicting the retention time and suitable for more kinds of chromatographic columns than the relative retention time method. Further studies shall be made for the standardization and practicability. Conclusion: The method of liner calibration with two reference substances can be used to assist the chromatographic peak identification

with a broad practical prospect.