四君子汤含药血清调控hTERT，TRF1，Tankyrase表达及对UCAC的影响

黄晓燕; 马媛萍; 李桂贤; 郑超伟; 李敏

doi:10.13422/j.cnki.syfjx.20180717

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四君子汤含药血清调控hTERT，TRF1，Tankyrase表达及对UCAC的影响

药理 | 更新时间：2024-01-04

- 四君子汤含药血清调控hTERT，TRF1，Tankyrase表达及对UCAC的影响
- Effect of Serum of Si Junzitang in Regulating hTERT, TRF1 and Tankyrase Expressions and UCAC
- 中国实验方剂学杂志 2018年24卷第8期页码：128-133
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81460714）;广西自然科学基金项目（2014GXNSFBA118183）;广西中医药管理局面上项目（GZPT1209）
- DOI：10.13422/j.cnki.syfjx.20180717
  中图分类号： R285
- 纸质出版日期：2018
- 稿件说明：
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黄晓燕, 马媛萍, 李桂贤, 等. 四君子汤含药血清调控hTERT，TRF1，Tankyrase表达及对UCAC的影响[J]. 中国实验方剂学杂志, 2018,24(8):128-133.

HUANG Xiao-yan, MA Yuan-ping, LI Gui-xian, et al. Effect of Serum of Si Junzitang in Regulating hTERT, TRF1 and Tankyrase Expressions and UCAC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 128-133.
黄晓燕, 马媛萍, 李桂贤, 等. 四君子汤含药血清调控hTERT，TRF1，Tankyrase表达及对UCAC的影响[J]. 中国实验方剂学杂志, 2018,24(8):128-133. DOI： 10.13422/j.cnki.syfjx.20180717.

HUANG Xiao-yan, MA Yuan-ping, LI Gui-xian, et al. Effect of Serum of Si Junzitang in Regulating hTERT, TRF1 and Tankyrase Expressions and UCAC[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 128-133. DOI： 10.13422/j.cnki.syfjx.20180717.

摘要

目的：应用四君子汤含药血清干预肠癌SW480细胞，观察人端粒酶逆转录酶（hTERT），端粒结合因子1（TRF1），端锚聚合酶（Tankyrase）mRNA及蛋白的表达变化，探讨其防治溃疡性结肠癌相关癌变（UCAC）的可能机制。方法：体外培养肠癌SW480细胞，分别予空白胎牛血清、四君子汤含药血清、美沙拉嗪含药血清干预，将经过干预后的SW480细胞，采用实时荧光定量PCR及蛋白免疫印迹法检测肠癌SW480细胞中hTERT，TRF1及Tankyrase mRNA和蛋白表达水平。结果：与空白血清组比较，四君子汤（2.16，4.32，8.64 g·kg-1）含药血清组和美沙拉嗪含药血清组均能降低hTERT，TRF1，Tankyrase mRNA表达（P < 0.05）；与美沙拉嗪含药血清组比较，四君子汤（4.32，8.64 g·kg-1）含药血清组明显降低hTERT，TRF1 mRNA表达（P < 0.05），四君子汤（2.16，4.32，8.64 g·kg-1）含药血清组Tankyrase mRNA表达明显升高（P < 0.05）。与空白血清组比较，四君子汤（2.16，4.32，8.64 g·kg-1）含药血清组和美沙拉嗪含药血清组hTERT，TRF1蛋白表达均降低（P < 0.05），美沙拉嗪含药血清组Tankyrase蛋白表达降低（P < 0.05）；四君子汤含药血清组Tankyrase蛋白表达呈下降趋势，但无统计学差异；与美沙拉嗪含药血清组比较，四君子汤（8.64 g·kg-1）含药血清组hTERT蛋白表达明显降低（P < 0.05），四君子汤（4.32，8.64 g·kg-1）含药血清组TRF1蛋白表达明显降低（P < 0.05），四君子汤（2.16 g·kg-1）含药血清组Tankyrase蛋白表达明显升高（P < 0.05）。结论：四君子汤含药血清可能通过下调hTERT，TRF1，Tankyrase表达，抑制癌细胞增殖，诱导癌细胞凋亡，达到防治UCAC的作用。

Abstract

Objective: To observe the gene and protein expressions of human telomerase reverse transcriptase (hTERT)

telomere binding factor 1 (TRF1)

endonel polymerase (Tankyrase) in colorectal cancer SW480 cells treated with Si Junzitang serum

in order to explore the possible mechanism of prevention and treatment of ulcerative colorectal cancer-associated carcinogenesis (UCAC). Method: Colorectal cancer SW480 cells were in vitro cultured and intervened with blank fetal bovine serum

different concentrations of Si Junzitang containing serum and mesalazine serum. After intervention

SW480 cells were detected by Real-time PCR and Western blot for the mRNA and protein expressions of hTERT

TRF1 and Tankyrase. Result: Compared with the blank serum group

the serum levels of hTERT

TRF1 and Tankyrase were decreased in the low

medium and high-dose group and the methadiazine serum group (P<0.05). Compared with the methacillin-containing serum group

the gene expression of Tankyrase in the serum group was significantly lower than that in the serum of Si Junzitang (P<0.05)

and the gene expressions of hTERT and TRF1 were significantly decreased in the high-dose group (P<0.05). Compared with the blank serum group

the serum levels of hTERT and TRF1 were decreased in Si Junzitang group

the middle and high-dose groups and the methadiazine serum group (P<0.05)

the protein expression of Tankyrase was decreased in the drug group (P<0.05)

the protein expression of Tankyrase in the Si Junzitang group was decreased

but with no significant difference. Compared with the methacillin-containing serum group

the protein expression of hTERT was significantly decreased in the high-dose group (P<0.05)

the protein expression of TRF1 was significantly decreased in the high-dose group (P<0.05)

the protein expression of Tankyrase in the serum-containing group was significantly lower than that in the low-dose group (P<0.05). Conclusion: The serum of Si Junzitang may inhibit the proliferation of cancer cells and induce the apoptosis of cancer cells by down-regulating the expressions of hTERT

TRF1 and Tankyrase.

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