Objective: To compare the hepatoprotective effects between the fruit and root of Gardenia jasminoides based on jaundice model

provide pharmacological basis for extending the medicinal parts of G.jasminoides

and provide theoretical support for its comprehensive utilization. Method: KM mice were randomly divided into 9 groups (n=10 in each group):normal group

model group

positive bifendate group (150 mg · kg-1)

high

middle and low dose G.jasminoides fruit groups

as well as high

middle and low dose G.jasminoides root groups. After 5 days of intragastric administration

α-naphthylisothiocyanate (ANIT) was used to establish icteric hepatitis models of mice. Meanwhile

the treatment was given. 48 h later

the alanine aminotransferase (ALT)

aspartic transaminase (AST)

γ-glutamyl transpeptidase (γ-GT)

total bilirubin (TBIL)

total bile acid (TBA)

superoxide dismutase (SOD)

glutathione(GSH)

and glutathione peroxidase (GSH-Px) levels were detected in serum and liver

and pathological changes were observed in mouse liver by hematoxylin-eosin (HE) staining; the mRNA expression levels of tumor necrosis factor-α (TNF-α)

interleukin-6 (IL-6)

bile salt export pump (BSEP)

sodium taurocholate cotransporting polypeptide (NTCP) and cholesterol 12α-hydroxylase (Cyp8b1) were detected in liver tissue by reverse-transcription polymerase chain reaction (RT-PCR). Result: As compared with the normal group

the levels of ALT

AST

γ-GT

TBIL

TBA in serum were significantly increased; SOD

GSH-Px

GSH activities in tissues were significantly decreased; NTCP and Cyp8b1 mRNA expression levels were significantly deceased; and TNF-α

IL-6 and BSEP mRNA expression levels were significantly increased in model group (P<0.01). As compared with the model group

the middle and high dose group of both fruit and root of G. jasminoides significantly reduced the levels of ALT

AST

γ-GT

TBIL

and TBA in serum

significantly enhanced the activities of SOD

GSH-Px and GSH in liver tissue

alleviated the pathological damage of liver

increased the mRNA expressions of NTCP and Cyp8b1

and decreased the mRNA expressions of TNF-α

IL-6 and BSEP (P<0.05

P<0.01); while the low dose groups only improved the single index or several indexes. Conclusion: The fruit and root of G.jasminoides had protective effect on ANIT induced jaundice model mice

and each dose of two sites had different effects on different indexes. The hepatoprotective mechanism of G. jasminoides fruit may be mainly related to the antioxidant activity and the inflammatory reaction

while the hepatoprotective effect of G. jasminoides root may be related to the metabolism of bile acid.