Objective: To investigate the efficacy of Shibuwan jiawei in treating premature ovarian failure (POF) with syndrome of yang deficiency of spleen and kidney and regulating the balance of Treg and Th17 and relevant cytokine level. Method: Totally 122 POF cases were randomly divided into control group and observation group by random number table

with 61 cases in each group. Control group was given the progesterone sequential therapy

orally administered with estradiol valerate tablets

1 g/time

qd

for 21 days

and also took progesterone pills

200 mg/time

qd

from the 12th days to the 21st days. In addition to the therapy of control group

observation group was treated with Shibuwan jiawei

1 dose/day

bid. The courses of treatment were 6 weeks for both groups. Syndromes of Yang deficiency of spleen and kidney

Kupperman scores

serum follicle-stimulating hormone (FSH)

estradiol (E2) and luteinizing hormone (LH) and efficacy were compared between two groups. Treg and Th17 in peripheral blood and serum interleukin (IL) -6

IL-21

interferon (IFN) -γ and transformation growth factor (TGF) -β1 in both groups were detected. Result: After treatment

scores of syndrome of Yang deficiency of spleen and kidney of observation group were obviously lower than those of control group (P<0.01). 3 weeks and 6 weeks after treatment

Kupperman score of observation group was remarkably lower than that of control group(P<0.01). After treatment

serum FSH and LH of observation group were evidently lower

while E2 was evidently higher than those of control group (P<0.01). Total efficacy rate of observation group was 88.33%

which was higher than 71.19% of control group (P<0.05). After treatment

Treg in peripheral blood of observation group was higher

while Th17 cell was lower than that of control group (P<0.01). Serum IL-6

IL-21

IFN-γ of observation group were evidently lower

while TGF-β1 was higher than those of control group (P<0.01). Conclusion: Shibuwan jiawei in treating syndrome of Yang deficiency of spleen and kidney of POF can alleviate clinical symptoms

adjust hormone level

and increase efficacy

which may be related to the adjustment of the balance of Treg and Th17 and relevant cytokines level.