金草消毒颗粒对-GalN/LPS致小鼠急性肝损伤保护的影响

王巍; 许立拔; 张卓; 张宇薇; 林彩霞; 高玲; 巫玲玲; 蒋伟哲

doi:10.13422/j.cnki.syfjx.20180736

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金草消毒颗粒对-GalN/LPS致小鼠急性肝损伤保护的影响

药理 | 更新时间：2024-01-04

- 金草消毒颗粒对-GalN/LPS致小鼠急性肝损伤保护的影响
- Effect of Jincao Xiaodu Granule on Acute Hepatic Injury in Mice induced by -GalN/LPS
- 中国实验方剂学杂志 2018年24卷第7期页码：108-113
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81260511）;广西科技计划项目（桂科AB16380187）
- DOI：10.13422/j.cnki.syfjx.20180736
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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王巍, 许立拔, 张卓, 等. 金草消毒颗粒对-GalN/LPS致小鼠急性肝损伤保护的影响[J]. 中国实验方剂学杂志, 2018,24(7):108-113.

WANG Wei, XU Li-ba, ZHANG Zhuo, et al. Effect of Jincao Xiaodu Granule on Acute Hepatic Injury in Mice induced by -GalN/LPS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 108-113.
王巍, 许立拔, 张卓, 等. 金草消毒颗粒对-GalN/LPS致小鼠急性肝损伤保护的影响[J]. 中国实验方剂学杂志, 2018,24(7):108-113. DOI： 10.13422/j.cnki.syfjx.20180736.

WANG Wei, XU Li-ba, ZHANG Zhuo, et al. Effect of Jincao Xiaodu Granule on Acute Hepatic Injury in Mice induced by -GalN/LPS[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(7): 108-113. DOI： 10.13422/j.cnki.syfjx.20180736.

摘要

目的：研究金草消毒颗粒（JCG，由肿节风、金钱草、白花蛇舌草、大青叶组成）对D-氨基半乳糖（D-GalN）/脂多糖（LPS）所致小鼠肝损伤的保护作用及机制。方法：JCG（1.7，3.4，6.8 g · kg-1）连续给药10 d后，采用腹腔注射给予D-GalN（700 mg · kg-1）和LPS（10 μg · kg-1）建立小鼠肝损伤模型。记录8 h小鼠存活率，生化检测小鼠血清丙氨酸氨基转移酶（ALT），天门冬氨酸氨基转移酶（AST），总胆红素（TBIL），尿素氮（BUN），肌酐（CREA），免疫球蛋白G（IgG），免疫球蛋白M（IgM）水平，酶联免疫吸附法（ELISA）检测白细胞介素-1β（IL-1β），白细胞介素6（IL-6），肿瘤坏死因子-α（TNF-α）水平，蛋白免疫印迹法（Western blot）检测肝组织一氧化氮合酶（iNOS），环氧合酶-2（COX-2），磷酸化型核转录因子-κB p65（p-NF-κB p65）的表达，采用苏木素-伊红（HE）染色观察肝组织的病理变化。结果：与模型组比较，金草消毒颗粒可提高小鼠存活率，降低血清ALT，AST，TBIL，BUN，CREA，IL-1β，IL-6，TNF-α水平（P<0.05，P<0.01），显著升高IgG，IgM水平（P<0.05，P<0.01），下调肝组织iNOS，COX-2，p-NF-κB p65表达（P<0.05，P<0.01），肝组织病理性损伤明显减轻。结论：金草消毒颗粒有很好的护肝作用，其机制可能是通过改善肝肾功能，减少促炎症因子水平，增强机体免疫功能，抑制肝组织iNOS，COX-2，p-NF-κB p65细胞因子的表达水平，达到保护肝细胞作用。

Abstract

Objective: To investigate the hepatoprotective effects and mechanisms of Jincao Xiaodu granule (JCG) on acute hepatic injury in mice induced by D-galactosamine (D-GalN)/lipopolysaccharide (LPS). Method: After administration of JCG (1.7

3.4

6.8 g · kg-1) for 10 consecutive days

the acute hepatic injury models in mice were induced by intraperitoneal injection of D-GalN (700 mg · kg-1)+LPS (10 μg · kg-1). The 8 h survival rate was recorded; the fasting serum levels of alanine transaminase (ALT)

aspartate transaminase (AST)

total bilirubin (TBIL)

blood urea nitrogen (BUN)

creatinine (CREA)

immunoglobulin G (IgG) and immunoglobulin M (IgM) were measured by biochemical test; the activity of tumor necrosis factor-α (TNF-α)

interleukin-1β (IL-1β) and IL-6 were determined by the enzyme-linked immunosorbent assay (ELISA); the protein expression levels of inducible nitric oxide synthase (iNOS)

cyclooxygenase-2 (COX-2)

and p-nuclear transcription factor-κB (NF-κB)p65 were analyzed by Western blot; and the pathological changes of liver tissues were observed by hematoxylin-eosin (HE) staining. Result: As compared with the model group

the survival rate was significantly increased; the levels of ALT

AST

TBIL

BUN

CREA

TNF-α

IL-1β and IL-6 in serum were significantly decreased (P<0.05

P<0.01); IgM and IgG were significantly increased (P<0.05

P<0.01); the expression levels of iNOS

COX-2 and p-NF-κB p65 were significantly down-regulated

and the pathological damages induced by D-GalN/LPS were significantly alleviated in JCG treatment group. Conclusion: JCG had good hepatoprotective effects

and its mechanism may be related to improving the hepatorenal function

decreasing the pro-inflammatory cytokine levels

up-regulating the immune function

as well as inhibiting the expression of iNOS

COX-2 and p-NF-κB p65.

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