Objective: In order to provide a certain reference value for the follow-up research and development of harmine

harmine-loaded ethosomes were prepared and its formula was optimized. Method: Harmine-loaded ethosomes were prepared by ethanol injection method.With the volume fraction of ethanol

the amount of phospholipids and the ratio of phospholipids/cholesterol (P/C) as factors

the particle size

Zeta potential and encapsulation efficiency as evaluation indexes

central composite design-response surface methodology was used to optimize the prescription of harmine-loaded ethosomes.The preliminary stability of the ethosomes was also evaluated. Result: The best prescription for harmine-loaded ethosomes were 0.03% of harmine

1.13% of lecithin

P/C ratio of 41.55:1 and 28% of ethanol volume fraction.The prepared ethosomes were round with uniform size and well dispersed.The encapsulation efficiency

particle size and Zeta potential of harmine-loaded ethosomes were 79.0%

124.8 nm and -57.1 mV

respectively. Conclusion: The preparation process of harmine-loaded ethosomes is simple.The prescription design is reasonable with good stability

which lays the foundation for the further study on the transdermal delivery of harmine-loaded ethosomes.