Objective: To establish the rapid molecular detection method for pathogen of aconiti downy mildew and provide the basis for the quarantine of the A. carmichaeli seedling and safety evaluation of the cultivated environmental land. Method: Pathogen DNA was extracted by fungal DNA kit and rDNA-ITS sequence of pathogen was amplified by ITS1/ITS4 universal primers of ribosomal internal transcribed spacer (ITS) in fungi; the polymerase chain reaction (PCR) product was recycled

purified and sequenced. The measured ITS sequences were then compared with the related ITS sequences searched in GeneBank; specific sequences were obtained by using DNAMAN and specific primers were designed and screened by using Primer Premier 5.0.With methods mentioned above

the rapid PCR method for identification of pathogen of Aconiti downy mildew was established. Result: The L1A/L1B primers with high sensitivity and specificity were selected from 8 pairs of designed primers in Primer Premier 5.0.L1A/L1B primers could be used to amplify 670 bp bright bands with detection value from the pathogen DNA

and they can be also used to detect whether the pathogen of Aconiti downy mildew was present in the Aconitum carmichaeli seedling

adult plant and cultivated soil. Conclusion: The primers selected above can be used to detect the pathogen more quickly

easily and effectively

and the established method can be used for the early detection and quarantine of A. carmichaeli seedling with downy mildew.