Objective: To establish the HPLC fingerprint of traditional Chinese medicine (TCM) compound Shensu Ⅱ(Shensu Ⅱ) and analyze its chemical components for its quality control and medicinal substance study.Method: Waters Symmetry C18 column (4.6 mm×250 mm

5 μm) was used with acetonitrile-0.1% phosphoric acid solution as the mobile phase for gradient elution. The detective wave length was 280 nm; the column temperature was set at 30℃; the injection volume was 10 μL; and the flow rate was 1.0 mL·min-1. 10 batches of Shensu Ⅱ samples were detected; their HPLC fingerprint was established by using Similarity Evaluation System for Chromatographic Fingerprint of TCM

and common peaks were identified through controls. UPLC-ESI-MS/MS was used to analyze the chemical components.Result: Fingerprints of 10 batches of Shensu Ⅱ were established and 15 common peaks were identified. The peaks were well separated

and the relative retention time of common peaks in each batch had RSD of<1.3% respectively; the similarity was more than 0.9 among different samples. Baicalin was identified as the characteristic peak

and 26 chemical components were identified as follows:ferulic acid

wogonin

calycosin

oroxylin A

chrysin-6-C-arabinose-8-C-glucoside

5

7-dyhydroxy-2'-methoxy flavone-7-O-glucuronide

5

7

2'

5'-tetrahydroxy-8

6'-dimethoxyflavanone

chrysin-6-C-glucose-8-C-arabinoside

tenaxin Ⅱ

trihydroxy-monomethoxy flavone

trihydroxy-dimethoxylflavone

formononetin

5

7

8-trihydroxyflavone

baicalein

norwogonin

baicalin

baicalin isomer

5

7

8-trihydroxy-6-methoxylflavone-7-O-glucuronide

chrysin

qroxylin A-7-O-glucuronide

wogonoside

astragalin

dyhydroxy-dimethoxylflavone

kumatakenin

trihydroxy-trihydroxyflavone

and skullcapflavone Ⅱ.Conclusion: The method can be used for the quality control of ShensuⅡ with good precision

high accuracy and reproducibility

providing basis for further studies on therapeutic efficacy of Shensu Ⅱ.