Objective: To study on the relationship between saponins and ecological factors of different habitats of Paris polyphylla var.chinensis. Method: The saponins ingredient of P.polyphylla var.chinensis from different samples were measured by HPLC. Nine ecological factors including temperature

altitude

moisture and light in different provinces were obtained from the ecological suitability database of the Digital Elevation Model and ArcGIS software packages. The regression equation of the ecological factors and PGGR

polyphyllin Ⅶ

H

I and V contents were established by SPSS 22.0 software using principal component and partial least squares regression method. Result: The total amount of Ⅶ

Ⅵ

Ⅱand I reached the minimum standard of 0.6% in the 2015 edition of Chinese Pharmacopoeia

the general quality of the herbs are higher growing in the Qingchuan

Hongya

Bazhong in Sichuan province. Ecological factors

such as annual sunshine time

maximum air temperature in July

average air temperature in July were a negative correlation with polyphyllin Ⅶ and PGGR

the minimum air temperature in January and average air temperature in January showed a positive correlation with the two saponins ingredients.Annual sunshine time and maximum air temperature in July and average air temperature in July were a positive correlation with polyphyllin H

I and V;elevation

relative humidity

annual precipitation

average air temperature in January showed a negative correlation with the three saponins ingredients. Conclusion: The results provided theoretical support for good quality cultivation of P. polyphylla var.chinensis.