Objective: To analyze and identify the main chemical constituents of Fangfeng Shaoyaotang by ultra performance liquid chromatography quadrupole time of flight mass spectrometry (HPLC-Q-TOF-MS/MS). Method: Agilent Eclipse XDB-C18 column (4.6 mm×250 mm

5 μm) was used with 0.1% formic acid solution-acetonitrile as the mobile phase for gradient elution. The column temperature was set at 25℃; the flow rate was 1 mL · min-1; the injection volume was 5 μL; and the detection wavelength was 254 nm. Both positive and negative electrospray ionization sources were used to detect and analyze the chromatographic effluent. Result: Based on molecular ion peaks and secondary mass spectrometry

and also by considering the standard mass spectrometry information and related literature reports

31 of 35 major peaks were finally identified

including 5 chromones

4 pinane monoterpenoid glycosides

15 flavonoids

which were the characteristic compounds of traditional Chinese medicine Saposhnikovia divaricata

Paeonia lactiflora and Scutellaria baicalensis respectively. Therefore

the main types of compounds in Fangfeng Shaoyaotang were determined. Conclusion: The established HPLC-Q-TOF-MS/MS method can be used to quickly analyze and identify the main chemical constituents of Fangfeng Shaoyaotang

which is of great significance to the further study of the pharmacological basis and its mechanism.