黄芪桂枝五物汤治疗糖尿病周围神经病变靶点预测与机制探讨

陈国铭; 钟晓莹; 赵金龙; 黄雁; 林洪荣; 梁妙珍; 刘韵韵; 於菁雯; 黄瑞麟; 刘敏

doi:10.13422/j.cnki.syfjx.20180821

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黄芪桂枝五物汤治疗糖尿病周围神经病变靶点预测与机制探讨

网络药理学 | 更新时间：2024-01-04

- 黄芪桂枝五物汤治疗糖尿病周围神经病变靶点预测与机制探讨
- Target Prediction and Mechanism of Huangqi Guizhi Wuwutang in Treatment of Diabetic Peripheral Neuropathy
- 中国实验方剂学杂志 2018年24卷第8期页码：214-222
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81373767）
- DOI：10.13422/j.cnki.syfjx.20180821
  中图分类号： R285
- 纸质出版日期：2018
- 稿件说明：
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陈国铭, 钟晓莹, 赵金龙, 等. 黄芪桂枝五物汤治疗糖尿病周围神经病变靶点预测与机制探讨[J]. 中国实验方剂学杂志, 2018,24(8):214-222.

CHEN Guo-ming, ZHONG Xiao-ying, ZHAO Jin-long, et al. Target Prediction and Mechanism of Huangqi Guizhi Wuwutang in Treatment of Diabetic Peripheral Neuropathy[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 214-222.
陈国铭, 钟晓莹, 赵金龙, 等. 黄芪桂枝五物汤治疗糖尿病周围神经病变靶点预测与机制探讨[J]. 中国实验方剂学杂志, 2018,24(8):214-222. DOI： 10.13422/j.cnki.syfjx.20180821.

CHEN Guo-ming, ZHONG Xiao-ying, ZHAO Jin-long, et al. Target Prediction and Mechanism of Huangqi Guizhi Wuwutang in Treatment of Diabetic Peripheral Neuropathy[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(8): 214-222. DOI： 10.13422/j.cnki.syfjx.20180821.

摘要

目的：通过网络药理学的方法对黄芪桂枝五物汤治疗糖尿病周围神经病变（DPN）的相关靶点进行预测与机制探讨。方法：利用中药网络药理学分析平台（TCMSP）数据库筛选出黄芪桂枝五物汤中有效成分和相关靶标蛋白；运用网络可视化软件Cytoscape 3.5.1构建黄芪桂枝五物汤有效成分-靶标相互作用网络进行拓扑学分析；借助STRING在线数据库进行蛋白质与蛋白质相互作用（PPI）网络的构建与分析；从Uniprot数据库中获取靶标蛋白的基因名称后，借助David数据库进行KEGG通路功能富集分析，研究黄芪桂枝五物汤在治疗DPN上的作用机制及原理。结果：黄芪桂枝五物汤的核心化合物有槲皮素（quercetin），山柰酚（kaempferol），β-谷甾醇（beta-sitosterol），7-O-甲基异丙醇胺（7-O-methylisomucronulatol），异鼠李素（isorhamnetin）等，核心靶标有前列腺素G/H合酶2（prostaglandin G/H synthase2），热休克蛋白HSP90（heat shock protein HSP90），核受体共激活因子2（uclear receptor coactivator2）等；PPI网络图显示黄芪桂枝五物汤有效成分中关键蛋白主要有C-Jun氨基端激酶（JUN），肿瘤蛋白p53（TP53），FOS，丝裂原活化蛋白激酶1（MAPK1），转录因子P65（RELA），白细胞介素-8（IL-8）等；KEGG结果表明黄芪桂枝五物汤主要作用与肿瘤坏死因子信号通路（TNF signaling pathway），Toll样受体信号通路（Toll-like receptor signaling pathway）等信号通路有关。结论：黄芪桂枝五物汤主要通过多种途径，多种信号通路作用于多种靶点发挥其药物功效，利用网络药理学通过多种数据库与统计分析软件可以有效预测与分析药物的潜在作用与起效机制。

Abstract

Objective: To predict and explore the target and mechanism of Huangqi Guizhi Wuwutang in the treatment of diabetic peripheral neuropathy (DPN) through systemic pharmacology. Method: The active constituents and related target proteins of Huangqi Guizhi Wuwutang were searched through TCMSP database. Cytoscape 3.5.1 was used to construct the topology analysis for the active ingredient-target interaction network of Huangqi Guizhi Wuwutang; STRING online database was used for the protein-protein interaction (PPI) network construction and analysis; target protein gene name was obtained from Uniprot database

David database was used for the analysis of KEGG pathway enrichment and the study for the mechanism and targets of Huangqi Guizhi Wuwutang in Treating DPN. Result: Quercetin

kaempferol

beta-sitosterol

7-O-methylisomucronulatol and isorhamnetin were the core compounds of Huangqi Guizhi Wuwutang. The core targets included Prostaglandin G/H synthase 2

Heat shock protein HSP 90

Nuclear receptor coactivator 2; PPI network diagram showed that the key components of the active ingredients of Huangqi Guizhi Wuwutang mainly included JUN

Tumor protein p53(TP53)

c-fos(FOS)

mitogenactivated protein kinase 1(MAPK1)

transcription factor p65(RELA)

interleukin-8(IL-8); KEGG results showed that the main effects of Huangqi Guizhi Wuwutang were correlated with TNF signaling pathway

Toll-like receptor signaling pathway and other signaling pathways. Conclusion: Huangqi Guizhi Wuwutang can exert the efficacy through a variety of channels

with multiple signaling pathways acting on a variety of targets. It can be effectively predicted and analyzed by systematic pharmacology through a variety of databases and statistical analysis software

so as to show its potential effect and mechanism of action.

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