Objective: A multi-target network and mechanism analysis of the main effective components of Tongxie Yaofang was carried out to illustrate the multi-component

multi-target and multi-pathway pharmacological mechanism of Tongxie Yaofang in treating irritable bowel syndrome. Method: Genes associated with irritable bowel syndrome were screened out through databases. Literatures and databases were retrieved to obtain the components and targets of Tongxie Yaofang. Functional annotation and pathway enrichment analysis of target genes were carried out by the Database for Annotation

Visualization and Integrated Discovery (DAVID) and Kyoto Encyclopedia of Genes and Genomes(KEGG) databases. The components

targets and disease pathways-related networks of Tongxie Yaofang were constructed and analyzed by Cytoscape software. Result: A total of 25 main active components of Tongxie Yaofang were screened out

involving 37 related targets. Based on this

the target component-effective target (C-T) network and the component-target-pathway (C-T-P) network were constructed

including 12 important components and 11 core targets and 26 crucial pathways. The functions of target genes involved gastrointestinal vascular smooth muscle

endocrine regulation and nerve immunity. Conclusion: Various effective components of Tongxie Yaofang act on multiple targets network

and then have a synergistic effect by multiple channels

including regulating inflammation

intestinal sensory function regulation

immune response

psychological adjustment and intestinal infection and flora disorder.