生长年限、采收期及施肥对唐古特大黄中儿茶素含量的影响

沈娜; 崔玉磊; 周华坤; 徐文华

doi:10.13422/j.cnki.syfjx.20180829

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生长年限、采收期及施肥对唐古特大黄中儿茶素含量的影响

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- 生长年限、采收期及施肥对唐古特大黄中儿茶素含量的影响
- Contents of Catechin in Radix and Rhizoma of at Different Growing Stages and Harvesting Stages and with Different Fertilizer Doses
- 中国实验方剂学杂志 2018年24卷第10期页码：38-42
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家星火计划项目（2015GA870007）;青海省应用基础研究项目（2017-ZJ-749）;青海省创新平台建设专项（2017-ZJ-Y20）;青海省自然科学基金面上项目（2016-ZJ-910）
- DOI：10.13422/j.cnki.syfjx.20180829
  中图分类号： S567.239
- 纸质出版日期：2018
- 稿件说明：
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沈娜, 崔玉磊, 周华坤, 等. 生长年限、采收期及施肥对唐古特大黄中儿茶素含量的影响[J]. 中国实验方剂学杂志, 2018,24(10):38-42.

SHEN Na, CUI Yu-lei, ZHOU Hua-kun, et al. Contents of Catechin in Radix and Rhizoma of at Different Growing Stages and Harvesting Stages and with Different Fertilizer Doses[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 38-42.
沈娜, 崔玉磊, 周华坤, 等. 生长年限、采收期及施肥对唐古特大黄中儿茶素含量的影响[J]. 中国实验方剂学杂志, 2018,24(10):38-42. DOI： 10.13422/j.cnki.syfjx.20180829.

SHEN Na, CUI Yu-lei, ZHOU Hua-kun, et al. Contents of Catechin in Radix and Rhizoma of at Different Growing Stages and Harvesting Stages and with Different Fertilizer Doses[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 38-42. DOI： 10.13422/j.cnki.syfjx.20180829.

摘要

目的：比较青海省东部农业区不同生长年限、采收期及施肥对唐古特大黄Rheum tanguticum药材中儿茶素含量的影响，确定这一区域采收唐古特大黄的最佳时间和施肥量。方法：设置5月，7月，10月3个采收期采收施加肥料（磷肥、钾肥）的二、三、四年生栽培唐古特大黄，样品的甲醇提取液高效液相色谱法（HPLC）分析采用Platisil SB-C18色谱柱（4.6 mm×250 mm，5 μm）；柱温25℃；以乙腈-0.1%甲酸为流动相进行梯度洗脱；进样量15 μL，流速0.8 mL·min-1；检测波长224 nm。结果：儿茶素在进样量0.424~10.60 μg时线性关系良好，相关系数为0.999 9。随生长年限的增加，儿茶素含量随之增加；同一年限不同采收期儿茶素平均含量5月份最高，7月份次之，10月份最低；施肥能够增加唐古特大黄中儿茶素的含量。结论：以儿茶素含量为采收指标时，宜采收三年生或四年生唐古特大黄，最佳采收期为5月，宜施加磷肥P2O5 45 kg·ha-1。三、四年生唐古特大黄5月份，7月份儿茶素平均含量均>20 mg·g-1。建议唐古特大黄中儿茶素质量分数不少于20%定为完善唐古特大黄药材质量控制的实验依据。

Abstract

Objective: To compare the content of catechin in radix and rhizoma of Rheum tanguticum at different growing stages and harvesting stages and with different fertilizer doses in Donggou Village

Huzu County

Qinghai province

in order to determine the best harvesting stage and fertilizer dose. Method: Three harvesting stages were designed in May

July and October every year

and two-year-old

three-year-old and four-year-old R. tanguticum radix and rhizoma were fertilized with phosphorus (superphosphate) and potassium (potassium sulfate) fertilizers. The methanol extracts were analyzed through HPLC. Platisil SB-C18 (4.6 mm×250 mm

5 μm) was adopted and eluted with acetonitrile-0.1% formic acid as mobile phase in a gradient mode. The column temperature was 25℃

the injection volume was 15 μL

the flow rate was 0.8 mL·min-1; the wavelength was 224 nm. Result: The linear ranges of catechin was 0.424-10.60 μg (r=0.999 9)

the content of catechin gradually increased with the rise of growing years; the average content of catechin came to the maximum in May

which was followed by July

and the least content was observed in October; the content of catechin in fertilizer group was higher than that of the control group. Conclusion: With the catechin content as the harvesting indicator

three or four-year-old R. tanguticum radix and rhizoma is suitable for harvesting

the optimal harvesting stages are May

and P2O5 45 kg·ha-1 was the most appropriate. Catechin content of three or four-year-old R. tanguticum radix and rhizoma exceeded 20 mg·g-1. Therefore

catechin content is suggested to be no less than 20%

which provides a basis for the quality control of R. tanguticum radix and rhizoma.

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