Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺

臧琛; 聂其霞; 王国华; 罗赣; 张保献; 李翠

doi:10.13422/j.cnki.syfjx.20180904

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Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺

药剂与炮制 | 更新时间：2024-01-04

- Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺
- Optimization of Vacuum Freeze-drying Technology for Dendrobii Officinalis Caulis by Box-Behnken Response Surface Methodology
- 中国实验方剂学杂志 2018年24卷第10期页码：15-20
- 作者机构：
- 作者简介：
- 基金信息：
  
  全国中药特色技术传承人才培训项目（L2016002）
- DOI：10.13422/j.cnki.syfjx.20180904
  中图分类号： R283.6
- 纸质出版日期：2018
- 稿件说明：
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臧琛, 聂其霞, 王国华, 等. Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺[J]. 中国实验方剂学杂志, 2018,24(10):15-20.

ZANG chen, NIE Qi-xia, WANG Guo-hua, et al. Optimization of Vacuum Freeze-drying Technology for Dendrobii Officinalis Caulis by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 15-20.
臧琛, 聂其霞, 王国华, 等. Box-Behnken响应面法优化铁皮石斛的真空冷冻干燥工艺[J]. 中国实验方剂学杂志, 2018,24(10):15-20. DOI： 10.13422/j.cnki.syfjx.20180904.

ZANG chen, NIE Qi-xia, WANG Guo-hua, et al. Optimization of Vacuum Freeze-drying Technology for Dendrobii Officinalis Caulis by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(10): 15-20. DOI： 10.13422/j.cnki.syfjx.20180904.

摘要

目的：采用响应面法优化铁皮石斛的真空冷冻干燥工艺，为其冷冻干燥工业化生产提供理论依据。方法：在单因素试验基础上，采用Box-Behnken响应面法，以总多糖质量分数为指标，考察升华干燥温度、升华干燥时间、真空度3个因素对铁皮石斛真空冷冻干燥工艺的影响，优化铁皮石斛的冻干工艺条件。采用HPLC建立铁皮石斛氨基酸类成分的指纹图谱，ODS-Hypersil色谱柱（0.46 cm×20 cm，5 μm），检测波长338 nm（0~37.5 min）和262 nm（37.5 min之后），柱温35℃。通过比较工艺优化后干燥样品与鲜品铁皮石斛指纹图谱的相似度，验证真空冷冻干燥工艺的可行性。结果：铁皮石斛最佳冷冻干燥工艺条件为升华干燥温度-16.4℃，升华干燥时间11.04 h，真空度85 Pa。在此条件下，干燥的铁皮石斛样品总多糖平均质量分数23.74%，与预测值基本一致，铁皮石斛干燥品与鲜品指纹图谱的相似度>0.90。结论：通过Box-Behnken响应面法建立的模型预测性较好，适用于铁皮石斛真空冷冻干燥工艺条件的优化。

Abstract

Objective: In order to provide a theoretical basis for mass production

the vacuum freeze-drying technology of Dendrobii Officinalis Caulis was optimized by Box-Behnken response surface methodology. Method: Based on single factor experiment

Box-Behnken response surface method was used to optimize vacuum freeze-drying technology of Dendrobii Officinalis Caulis with the content of total polysaccharides as index.The fingerprint of amino acids in Dendrobii Officinalis Caulis was established by HPLC.HPLC was conducted on ODS-Hypersil column (0.46 cm×20 cm

5 μm)with gradient elution;the detection wavelength was set at 338 nm before 37.5 min

then changed to 262 nm.The similarity of fingerprints between samples dried by the optimized technology and fresh samples was calculated. Result: The best freeze-drying process conditions of Dendrobii Officinalis Caulis were as follows:the sublimation drying temperature of -16.4℃

the sublimation drying time of 11.04 h

vacuum degree of 85 Pa.Under these conditions

the content of total polysaccharides in Dendrobii Officinalis Caulis was 23.74%

its tested value and predicted value were basic identical.Compared with the fresh samples

the similarity of fingerprint of dried products was more than 0.90. Conclusion: Model established by Box-Behnken response surface methodology has good predictability

which is suitable for optimizing the vacuum freeze-drying technology of Dendrobii Officinalis Caulis.

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