黄芩ITS2条形码数据库构建及其种子的DNA条形码鉴定方法建立

刘金欣; 魏妙洁; 李耿; 程硕涵; 赵春颖; 阿里穆斯; 石林春

doi:10.13422/j.cnki.syfjx.20180906

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黄芩ITS2条形码数据库构建及其种子的DNA条形码鉴定方法建立

资源与鉴定 | 更新时间：2024-01-04

- 黄芩ITS2条形码数据库构建及其种子的DNA条形码鉴定方法建立
- Construction of ITS2 Barcode Database of Scutellariae Radix and Establishment of DNA Barcode Identification Method for Its Seeds
- 中国实验方剂学杂志 2018年24卷第9期页码：37-45
- 作者机构：
- 作者简介：
- 基金信息：
  
  河北省三三三人才项目（A2017002090）;河北省教育厅青年拔尖人才项目（BJ201602）;河北省自然科学基金项目（H2017406031）;河北省中医药管理局项目（2017077）;河北省高校重点学科建设项目（冀教高[2013]4号）
- DOI：10.13422/j.cnki.syfjx.20180906
  中图分类号： R282.5
- 纸质出版日期：2018
- 稿件说明：
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刘金欣, 魏妙洁, 李耿, 等. 黄芩ITS2条形码数据库构建及其种子的DNA条形码鉴定方法建立[J]. 中国实验方剂学杂志, 2018,24(9):37-45.

LIU Jin-xin, WEI Miao-jie, LI Geng, et al. Construction of ITS2 Barcode Database of Scutellariae Radix and Establishment of DNA Barcode Identification Method for Its Seeds[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(9): 37-45.
刘金欣, 魏妙洁, 李耿, 等. 黄芩ITS2条形码数据库构建及其种子的DNA条形码鉴定方法建立[J]. 中国实验方剂学杂志, 2018,24(9):37-45. DOI： 10.13422/j.cnki.syfjx.20180906.

LIU Jin-xin, WEI Miao-jie, LI Geng, et al. Construction of ITS2 Barcode Database of Scutellariae Radix and Establishment of DNA Barcode Identification Method for Its Seeds[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(9): 37-45. DOI： 10.13422/j.cnki.syfjx.20180906.

摘要

目的：通过构建黄芩核糖体DNA内转录间隔区2（ITS2）条形码数据库，建立黄芩种子DNA条形码鉴定新方法，保障黄芩种子基原准确。方法：收集黄芩对照药材、基原植物、生药材、公共数据库及其常见代用品的ITS2序列，构建黄芩DNA条形码数据库；收集62份市售黄芩种子样品，每份种子获取3~4条ITS2序列，基于BLAST方法、遗传距离法和邻接（NJ）系统发育树法进行物种鉴定。结果：共获得101条黄芩及其代用品的ITS2序列。黄芩的种内序列变异小于种间序列变异，黄芩、甘肃黄芩和粘毛黄芩在NJ系统发育树上分别聚为独立的支，可相互区分。共获得195条黄芩种子的ITS2序列，DNA条形码鉴定结果表明193条为黄芩序列，2条为真菌序列。结论：黄芩ITS2条形码数据库稳定可靠，可满足黄芩种子DNA条形码鉴定的需求。DNA条形码技术可用于黄芩种子的物种鉴定。

Abstract

Objective: In order to guarantee the seed base of Scutellariae Radix is accurate

an internal transcribed spacer 2 (ITS2) barcode reference library of Scutellariae Radix has been constructed and then a species identification method of Scutellariae Radix seeds based on DNA barcoding technology has been established. Method: ITS2 sequences were obtained from Scutellariae radix reference medicinal materials

original plant

crude medicinal materials

public database

and its substitutes.Totally 62 Scutellariae Radix seed specimens were collected from markets.For each Scutellariae Radix seed specimen

3-4 ITS2 sequences were obtained and then used for species assignment by BLAST method

genetic distance method and neighbor-joining phylogenetic tree method. Result: In total

101 ITS2 sequences of Scutellariae Radix and its substitutes have been obtained.The data analysis result showed that the intraspecific distance of Scutellariae Radix was less than its interspecific distance.The three species of Scutellaria baicalensis

S. rehderiana and S. viscidula were showed separated branch in the neighbor-joining tree.Totally 195 ITS2 sequences have been obtained from Scutellariae Radix seeds

the identification results showed that 193 sequences were Scutellariae Radix and 2 sequences were fungi contaminations. Conclusion: The ITS2 barcode database of Scutellariae Radix is stable and reliable

it can satisfy the demand of DNA barcoding analysis of Scutellariae Radix seeds.

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