Objective: To identify the origins of Gastrodiae Rhizoma by nonlinear chemical fingerprint technology and HPLC

make a quantitative analysis of gastrodin and global active components in Gastrodiae Rhizoma. Method: Gastrodiae Rhizoma from Yunnan

Hubei

Guizhou and Northeast China were collected and identified by nonlinear fingerprint and HPLC fingerprint. With Gastrodiae Rhizoma sample as oscillation substrate

H2SO4+MnSO4+NaBrO3+CH3COCH3+sample compositions was taken as the oscillation system to establish the nonlinear chemical fingerprint of Gastrodiae Rhizoma. Based on the significant differences of the nonlinear fingerprint and system similarity model

Gastrodiae Rhizoma from different areas was identified successfully

and their global active components were detected. Meanwhile

HPLC fingerprints of Gastrodiae Rhizoma from 4 different origins were established. Then a fuzzy cluster analysis was made to study their quality difference

and gastrodin was detected. Result: The classification results by nonlinear chemical fingerprint and HPLC fingerprint were consistent

and can trace to the origins of Gastrodiae Rhizoma. The relative contents of functional components in Gastrodiae Rhizoma produced from different regions showed different quality. HPLC fingerprint and non-linear chemical fingerprint were combined to detect each single active component and obtain the result of global content of active components. Conclusion: This study provides a new economical and rapid method for identification of origins and quality evaluation of Gastrodiae Rhizoma and new ideas to represent active components in Gastrodiae Rhizoma.