灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-B通路的影响

刘亭; 刘香香; 陈亭亭; 刘帆; 侯佳; 孙佳; 王爱民

doi:10.13422/j.cnki.syfjx.20180930

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灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-B通路的影响

药理 | 更新时间：2024-01-04

- 灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-B通路的影响
- Effect of Combined Formula of Erigerontis Herba and Paeoniae Radix Rubra on Cerebral Ischemia Reperfusion Injury and NF-B Pathway in Rats
- 中国实验方剂学杂志 2018年24卷第9期页码：111-115
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目（81760699，81260636）;贵州省科学技术厅人才团队项目（黔科合平台人才[2016]5613\5677）;贵州省教育厅项目（黔教合协同创新字[2013]04）
- DOI：10.13422/j.cnki.syfjx.20180930
  中图分类号： R285.5
- 纸质出版日期：2018
- 稿件说明：
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刘亭, 刘香香, 陈亭亭, 等. 灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-B通路的影响[J]. 中国实验方剂学杂志, 2018,24(9):111-115.

LIU Ting, LIU Xiang-xiang, CHEN Ting-ting, et al. Effect of Combined Formula of Erigerontis Herba and Paeoniae Radix Rubra on Cerebral Ischemia Reperfusion Injury and NF-B Pathway in Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(9): 111-115.
刘亭, 刘香香, 陈亭亭, 等. 灯盏细辛和赤芍配伍组方对大鼠脑缺血再灌注损伤的保护作用及对NF-B通路的影响[J]. 中国实验方剂学杂志, 2018,24(9):111-115. DOI： 10.13422/j.cnki.syfjx.20180930.

LIU Ting, LIU Xiang-xiang, CHEN Ting-ting, et al. Effect of Combined Formula of Erigerontis Herba and Paeoniae Radix Rubra on Cerebral Ischemia Reperfusion Injury and NF-B Pathway in Rats[J]. Chinese journal of experimental traditional medical formulae, 2018, 24(9): 111-115. DOI： 10.13422/j.cnki.syfjx.20180930.

摘要

目的：探讨灯盏细辛和赤芍配伍组方（辛芍）对大鼠中动脉阻断（MCAO）局灶脑缺血再灌注损伤的保护作用及其机制。方法：随机将SPF级SD大鼠分为假手术组、模型组、尼莫地平组（12.6 mg · kg-1）和辛芍组（12.5 g · kg-1）。给药组连续灌胃给药3 d，然后选用改良Zea Longa方法建立大鼠局灶脑缺血再灌注损伤模型，术后再连续给药7 d。采用Zea Longa5分制法对各组大鼠进行神经行为学判别；用2，3，5-氯化三苯基四氮唑（TTC）染色法测定脑梗死面积；用酶联免疫吸附法（ELISA）测定白细胞介素-6（IL-6），白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的含量；并用蛋白质免疫印迹法（Western blot）检测p65和IκBα的磷酸化水平。结果：与假手术组比较，模型组出现明显神经功能缺失体征（P<0.01），脑梗死面积明显增加（P<0.01），说明大鼠局灶脑缺血再灌注损伤模型构建成功。与模型组比较，辛芍组方能明显改善脑缺血再灌注损伤模型大鼠的神经行为功能（P<0.05），明显降低脑梗死面积比（P<0.05），显著降低IL-6，IL-1β和TNF-α的水平（P<0.01），并显著降低p65和IκBα的磷酸化水平（P<0.01）。结论：辛芍具有减轻大鼠脑缺血再灌注损伤的作用，其作用机制可能与抑制核转录因子-κB（NF-κB）通路激活相关。

Abstract

Objective: To investigate the protective effect and mechanism of combined formula of Erigerontis Herba and Paeoniae Radix Rubra (Xinshao) on cerebral ischemia reperfusion injury in rats. Method: The rats were randomly divided into four groups:sham operation group

model group

nimodipine group (12.6 mg · kg-1) and Xinshao group (12.5 g · kg-1). Rats were administrated with drugs for 3 days; then the cerebral ischemia-reperfusion model was established by improved Zea longa method. After surgery

the rats were administrated with drugs for 7 days. The neurological behavior was evaluated by Zea Longa method.triphenyltetrazolium Chloride(TTC) staining was performed to determine the area of cerebral infarction. interleukin-6(IL-6)

IL-1β and tumor necrosis factor-α(TNF-α) levels in serum were examined by enzyme-linked immunosurbent assay(ELISA). The levels of phosphorylation of p65 and IκBα were detected by Western blot. Result: Compared with sham operation group

the score of neurological behavior and the percentage of infarct area were significantly increased in model group

which indicated the successful establishment of the MACO model. Xinshao could significantly ameliorate the neurological behavior (P<0.05)

and decrease the percentage of infarct area (P<0.05). Compared with model group

the levels of IL-6

IL-1β and TNF-α in serum were significantly lowered in Xinshao group (P<0.01). Western blot analysis showed that Xinshao could reduce the phosphorylation levels of p65 and IκBα (P<0.01). Conclusion: Xinshao has a protective effect on cerebral ischemia reperfusion injury in rats

which may be associated with the inhibition of nuclear transcription factor-κB(NF-κB) pathway.

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