Objective: To study the effect of Alismatis Rhizoma polysaccharide (AP) on glucose-lipid metabolism as well as the mRNA and protein expression of Adipo R2 and PPARα in diabetic rats

and discuss the potential mechanism. Method: Diabetic rats model was induced by intraperitoneal injection of streptozotocin (STZ)

and the diabetic rats were randomly divided into six groups

including normal group

model group

Pioglitazone hydrochloride group

AP high

medium and low (400

200

100 mg ·kg-1) dose groups

n=8 in each group. The drug administration was given for 30 days. The body weight

liver weight

oral glucose tolerance test (OGTT)

fasting blood glucose (FBG)

fasting insulin (FINS)

glycosylated serum protein (GSP)

glycosylated hemoglobin (HbA1c)

serum total cholesterol (TC)

triglyceride (TG)

low density lipoprotein (LDL)

high density lipoprotein (HDL) and liver histology index were compared. Immunohistochemical method was used to observe the level of insulin-like growth factors (IGF). The protein expression levels of Adipo R2 and PPARα in liver tissues of different groups were measured with Western blot

and the mRNA expression levels were measured with Real-time PCR. Result: As compared with the model group

AP could significantly reduce the body weight and liver weight of diabetic rats(P<0.01)

as well as the levels of FBG

HbA1c and GSP

improve the indexes of TC

TG

LDL and HDL(P<0.05

P<0.01)

and regulate the mRNA and protein expression levels of Adipo R2 and PPARα in liver tissues(P<0.05

P<0.01). Conclusion: AP can significantly adjust the glucolipid metabolism in diabetic rats

and its potential mechanism is related to the regulation of the expression levels of Adipo R2 and PPARα

with certain prevention and treatment effectfor diabetes complications and fatty accumulation in liver tissues.